Veterinary Archives, Vol. 80 No. 4, 2010.
Original scientific paper
The developmental competence of bovine immature oocytes and quality of embryos derived from slaughterhouse ovaries or live donors by ovum pick up
Martina Karadjole
orcid.org/0000-0003-2718-3348
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Iva Getz
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Marko Samardžija
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Nino Maćešić
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Mario Matković
; Center for Reproduction and Animals Breeding of Croatia, Zagreb, Croatia
Zdenko Makek
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Tugomir Karadjole
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Goran Bačić
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Tomislav Dobranić
; Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Marko Poletto
; Postgraduate specialist student, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Abstract
The aim of this research was to compare the developmental competence of oocytes recovered from slaughterhouse ovaries (SO) and those recovered in vivo by ovum pick up (OPU). OPU was performed in 6 donor heifers synchronized with PGF2α and stimulated with pFSH, twice a day for two days. OPU was repeated once a week for six consecutive weeks. The control group consisted of slaughterhouse-derived oocytes. The number of aspirated follicles, retrieved oocytes and oocyte recovery rate were recorded. Oocytes of grade 1 and 2 were matured, fertilized and cultured in vitro in SOFaaBSA until day 9. The cleavage rates on day 2, the total number of morulas and blastocysts on day 7 and the numbers of hatched blastocysts on day 9 were recorded. Differential staining of the inner cell mass (ICM) and trophectoderm cells (TE) were performed on a random sample of day 7 blastocysts (n = 24). Significantly more follicles were aspirated from SO (19.3 versus 11.5, P<0.05). This was reflfl ected in a significantly higher number of oocytes collected from the same group (12.6 versus 6.5, P<0.05). A higher proportion of OPU-derived oocytes reached the morula/blastocyst stage at day 7 (44.7% vs. 29.9%, P<0.05) and hatched blastocyst stage at day 9 (35.6% vs. 16.8%, P<0.05). OPU-derived embryos displayed significantly higher number (P<0.05) of ICM cells which was also reflected to higher proportion of ICM cells. The results demonstrate that oocytes recovered in vivo after OPU are more competent to develop to the blastocyst stage than those derived from slaughterhouse ovaries.
Keywords
heifer; oocyte; developmental competence
Hrčak ID:
58459
URI
Publication date:
28.7.2010.
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