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The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens

Marina Tišljar
Željko Grabarević
Branka Artuković
Petar Džaja
Stipe Ćenan
Tajana Amšel Zelenika
Ross G. Cooper
Marija Dinarina-Sablić

Puni tekst: engleski, pdf (576 KB) str. 547-556 preuzimanja: 307* citiraj
APA 6th Edition
Tišljar, M., Grabarević, Ž., Artuković, B., Džaja, P., Ćenan, S., Amšel Zelenika, T., ... Dinarina-Sablić, M. (2011). The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens. Collegium antropologicum, 35 (2), 547-556. Preuzeto s https://hrcak.srce.hr/69149
MLA 8th Edition
Tišljar, Marina, et al. "The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens." Collegium antropologicum, vol. 35, br. 2, 2011, str. 547-556. https://hrcak.srce.hr/69149. Citirano 29.07.2021.
Chicago 17th Edition
Tišljar, Marina, Željko Grabarević, Branka Artuković, Petar Džaja, Stipe Ćenan, Tajana Amšel Zelenika, Ross G. Cooper i Marija Dinarina-Sablić. "The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens." Collegium antropologicum 35, br. 2 (2011): 547-556. https://hrcak.srce.hr/69149
Harvard
Tišljar, M., et al. (2011). 'The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens', Collegium antropologicum, 35(2), str. 547-556. Preuzeto s: https://hrcak.srce.hr/69149 (Datum pristupa: 29.07.2021.)
Vancouver
Tišljar M, Grabarević Ž, Artuković B, Džaja P, Ćenan S, Amšel Zelenika T i sur. The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens. Collegium antropologicum [Internet]. 2011 [pristupljeno 29.07.2021.];35(2):547-556. Dostupno na: https://hrcak.srce.hr/69149
IEEE
M. Tišljar, et al., "The Impact of L-NAME and L-arginine Chronic Toxicity Induced Lesions on Ascites – Pulmonary Hypertension Syndrome Development in Broiler Chickens", Collegium antropologicum, vol.35, br. 2, str. 547-556, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/69149. [Citirano: 29.07.2021.]

Sažetak
The impact of L-arginine (LA), a precursor for synthesis of nitric oxide (NO), and N-w -nitro-L-arginine methyl ester
(L-NAME, LN), a non-selective inhibitor of the enzyme producing nitric oxide (nitric oxide synthase; NOS) chronic toxicity
induced lesions on Ascites – Pulmonary hypertension syndrome (PHS) development was investigated in 140 one-day-
-old male broiler chickens (ROSS) during the first 5 weeks of life. Every second day the animals were treated intraperitoneally
(ip) with L-NAME (10 mg/kg of body weight; BW), L-arginine (100 mg/kg BW), L-arginine and L-NAME in
combination (100 mg/kg BW and 10 mg/kg BW, respectively), and with physiological saline (0.90% w/v of NaCl; 0.5
mL/kg BW). Seven birds from each group were euthanized every week. The histopathological examination of the heart,
the liver, the lungs, the blood vessels and the lymphoid organs, was performed. Also the organ index values were determined.
At the end of the experiment the pre-ascitic condition or ascites – PHS was confirmed in five dead animals in the
L-NAME-treated group. In the same group the edema was the most prominent histopathological change confirmed in the
heart and in the lungs of the sacrificed chickens. In L-arginine-treated group the congestion and the haemorrhages were
the striking changes in the same organs with the highest degree in the last two weeks of trial. While the focal disruption
of myocardiofibriole and hepatocytes were predominant lesions in L-NAME-treated chickens (5thand 4th weeks, respectively),
in L-NAME/L-arginine-treated group only the mild focal myocardial degeneration was seen. According to the
most of the results of present investigation, it was concluded that the consecutive treatment with L-NAME provoked
ascites – PHS, while L-arginine has protective effect in this animal model of disease.

Ključne riječi
broiler; Ascites – Pulmonary hypertension syndrome (PHS); nitric oxide; L-NAME; L-arginine; histopathology; organ index

Hrčak ID: 69149

URI
https://hrcak.srce.hr/69149

Posjeta: 649 *