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Original scientific paper

RAZLIKOVANJE HRVATSKIH SORTI JEČMA SDS-POLIAKRILAMID ELEKTROFOREZOM U GRADIJENT GELU I ELEKTROFORETSKIM FOKUSIRANJEM HORDEINA EKSTRAHIRANIH IZ SUHOGA ZRNJA I ZELENOGA SLADA

Ivica Strelec   ORCID icon orcid.org/0000-0003-3999-8499 ; Sveučilište J.J. Strossmayer, Prehrambeno-tehnološki fakultet u Osijeku, Franje Kuhača 20, 31000 Osijek,Hrvatska
Elzabeta Has-Schön ; Sveučilište J.J. Strossmayer, Odjel za biologiju, Gajev trg 6,, 31000 Osijek,Hrvatska
Ljubinka Vitale ; Institut Ruđer Bošković, Bijenička cesta 54, HR-10000 Zagreb, Hrvatska

Fulltext: english, pdf (218 KB) pages 23-27 downloads: 494* cite
APA 6th Edition
Strelec, I., Has-Schön, E. & Vitale, Lj. (2011). DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS. Poljoprivreda, 17 (1), 23-27. Retrieved from https://hrcak.srce.hr/69540
MLA 8th Edition
Strelec, Ivica, et al. "DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS." Poljoprivreda, vol. 17, no. 1, 2011, pp. 23-27. https://hrcak.srce.hr/69540. Accessed 22 Jan. 2020.
Chicago 17th Edition
Strelec, Ivica, Elzabeta Has-Schön and Ljubinka Vitale. "DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS." Poljoprivreda 17, no. 1 (2011): 23-27. https://hrcak.srce.hr/69540
Harvard
Strelec, I., Has-Schön, E., and Vitale, Lj. (2011). 'DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS', Poljoprivreda, 17(1), pp. 23-27. Available at: https://hrcak.srce.hr/69540 (Accessed 22 January 2020)
Vancouver
Strelec I, Has-Schön E, Vitale Lj. DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS. Poljoprivreda [Internet]. 2011 [cited 2020 January 22];17(1):23-27. Available from: https://hrcak.srce.hr/69540
IEEE
I. Strelec, E. Has-Schön and Lj. Vitale, "DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS", Poljoprivreda, vol.17, no. 1, pp. 23-27, 2011. [Online]. Available: https://hrcak.srce.hr/69540. [Accessed: 22 January 2020]

Abstracts
Ispitana je mogućnost primjene gel elektroforeze i izoelektričnoga fokusiranja hordeina za razlikovanje šest sorti ozimoga dvorednoga ječma (Angora, Sladoran, Rodnik, Rex, Martin, Barun). Hordeini ekstrahirani iz suhoga zrnja i zelenoga slada ječma razdvajani su pomoću SDS- poliakrilamid-gel elektroforeze u gradijentu od 8-18% T i pomoću izoelektričnoga fokusiranja u gradijentima pH 3,5-9,5 i pH 5,5-8,5. U svim pokusima elektroforetskoga razdvajanja ekstrakti proteina iz suhoga zrna davali su bolje rezultate od onih iz zelenoga slada. Sorte Angora, Sladoran i Martin mogle su se razlikovati međusobno i od drugih sorti po elektroforetskome profilu svojih hordeina dobivenih SDSPAGE u gradijent gelu (8-18% T), a sorte Angora, Sladoran, Martin i Rodnik pomoću IEF u pH gradijentu 5,5-8,5.

Keywords
razlikovanje sorti ječma; SDS-PAGE u gradijent gelu; hordeini; izoelektrično fokusiranje

Hrčak ID: 69540

URI
https://hrcak.srce.hr/69540

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