Izvorni znanstveni članak
Y-short tandem repeat haplotype and paternal lineage of the Ezhava population of Kerala, south India
Parvathy Seema Nair
; Center for Biotechnology and Nanotechnology, Department of Biotechnology and Biochemical Engineering, Sree Buddha College of Engineering, Kerala, India
Aswathy Geetha
; Center for Biotechnology and Nanotechnology, Department of Biotechnology and Biochemical Engineering, Sree Buddha College of Engineering, Kerala, India
Chippy Jagannath
; Center for Biotechnology and Nanotechnology, Department of Biotechnology and Biochemical Engineering, Sree Buddha College of Engineering, Kerala, India
Sažetak
Aim To analyze the haplotype of the Ezhava population of
Kerala, south India, using 8 short tandem repeat (STR) loci
on the Y chromosome and trace the paternal genetic lineage
of the population.
Methods Whole blood samples (n = 104) were collected
from unrelated healthy men of the Ezhava population over
a period of one year from October 2009. Genomic DNA
was extracted by salting out method. All samples were
genotyped for the 8 Y-STR loci by the AmpFiSTR Y-filer PCR
Amplification Kit. The haplotype and allele frequencies
were determined by direct counting and analyzed using
Arlequin 3.1 software, and molecular variance was calculated
with the Y-chromosome haplotype reference database
online analysis tool, www.yhrd.org.
Results Among the 104 examined haplotypes, we found
98 unique ones. The average gene diversity was 0.669,
with the highest diversity of 0.9462 observed for the biallelic
Y-STR marker DYS 385. The allele frequency among
DYS loci varied between 0.0096 and 0.75. Out of the 104
haplotypes, 10 were identical to the Jat Sikh population of
Punjab, which is the greatest number among the Indian
populations, and 4 to the Turkish population, which is the
greatest number among the European populations. According
to the allele frequency of Y-STR, the Ezhavas were
genetically more similar to the Europeans (60%) than to
the East Asians (40%).
Conclusion The vast majority of haplotypes were observed
only once, reflecting the enormous genetic heterogeneity
of the Ezhavas. Based on the genotype, the Ezhavas
showed more resemblance to Jat Sikh population of
Punjab and the Turkish populations than to the East Asians,
hence indicating a paternal lineage of European origin.
Ključne riječi
Hrčak ID:
71444
URI
Datum izdavanja:
15.6.2011.
Posjeta: 1.567 *