APA 6th Edition Pernek, M., Županić, M., Diminić, D. i Cech, T. (2011). Vrste roda Phytophthora na bukvi i topolama u Hrvatskoj. Šumarski list, 135 (13), 130-137. Preuzeto s https://hrcak.srce.hr/72321
MLA 8th Edition Pernek, Milan, et al. "Vrste roda Phytophthora na bukvi i topolama u Hrvatskoj." Šumarski list, vol. 135, br. 13, 2011, str. 130-137. https://hrcak.srce.hr/72321. Citirano 02.07.2020.
Chicago 17th Edition Pernek, Milan, Miljenko Županić, Dinko Diminić i Thomas Cech. "Vrste roda Phytophthora na bukvi i topolama u Hrvatskoj." Šumarski list 135, br. 13 (2011): 130-137. https://hrcak.srce.hr/72321
Harvard Pernek, M., et al. (2011). 'Vrste roda Phytophthora na bukvi i topolama u Hrvatskoj', Šumarski list, 135(13), str. 130-137. Preuzeto s: https://hrcak.srce.hr/72321 (Datum pristupa: 02.07.2020.)
Vancouver Pernek M, Županić M, Diminić D, Cech T. Vrste roda Phytophthora na bukvi i topolama u Hrvatskoj. Šumarski list [Internet]. 2011 [pristupljeno 02.07.2020.];135(13):130-137. Dostupno na: https://hrcak.srce.hr/72321
IEEE M. Pernek, M. Županić, D. Diminić i T. Cech, "Vrste roda Phytophthora na bukvi i topolama u Hrvatskoj", Šumarski list, vol.135, br. 13, str. 130-137, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/72321. [Citirano: 02.07.2020.]
Sažetak The genus Phytophthora, belongs to the Oomycota, parasitizes on many species of woody plants, causing decay of roots which is often followed by climatic extremes. Phytophthora spores can survive in soil for several years waiting for optimal conditions for germination, zoosporangium is formed, from which zoospores are released. The zoospores are chemotactic swimmers which actively find tree fine roots that become infected. The disease develops over a long number of years, but certain factors can significantly speed up the decline of trees. Bleeding lesions on the trunk (Figure 1–3), increasing of the amount of dead branches, and lack of lateral shoots are typical symptoms ofPhytophthorainfection which was registered on two different tree species in Croatia (Fagus sylvaticaandPopulus x euroamericana). The consequence was a reduction of surface of leaves or the decline of vitality, and ultimately death of the tree. Given the importance of the problem in the world, and the potential dangers of Croatian forests, the aim of this study was to determine the presence of Phytophthora species in Croatia, and to assess the threat of their expansion. Investigations were carried out in Bjelovar and Osijek area in beech and poplar euro-american clones (Table 1). Samples of bark and soil were taken from trees with clear symptoms of the disease, and analysed in the laboratory. Parts of the samples were transferred to culture media (PDA, V8), and molecular analysis of suspected isolates was performed (Figure 5). Soil samples were processed by immersing in water, and pears, young leaves of oak, rhododendron young leaves and fresh apples were used as baits. The obtained samples were processed by surface sterilization and placed on several types of culture media, as mentioned above. Obtained pure cultures (isolates) were analyzed by molecular tehnics.
The results show that the presence of P. cambivorawas confirmed on beech andP. cambivoraandP. ganopodyideson poplar. The occurrence of P. cambivorais related to changes of extreme dry and wet periods (Figure 6). The results on poplars shows that clone 275/81 have been significantly damaged by species of Phytophthora.