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Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera

Polona Žigon ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Katja Lakota ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Saša Čučnik ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Tinka Švec ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Aleš Ambrožič ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Snežna Sodin-Šemrl ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia
Tanja Kveder ; University Medical Centre Ljubljana, Department of Rheumatology, Immunology Laboratory, Ljubljana, Slovenia

Puni tekst: engleski, pdf (466 KB) str. 694-702 preuzimanja: 773* citiraj
APA 6th Edition
Žigon, P., Lakota, K., Čučnik, S., Švec, T., Ambrožič, A., Sodin-Šemrl, S. i Kveder, T. (2011). Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera. Croatian Medical Journal, 52 (6), 694-702. Preuzeto s https://hrcak.srce.hr/78199
MLA 8th Edition
Žigon, Polona, et al. "Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera." Croatian Medical Journal, vol. 52, br. 6, 2011, str. 694-702. https://hrcak.srce.hr/78199. Citirano 16.06.2021.
Chicago 17th Edition
Žigon, Polona, Katja Lakota, Saša Čučnik, Tinka Švec, Aleš Ambrožič, Snežna Sodin-Šemrl i Tanja Kveder. "Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera." Croatian Medical Journal 52, br. 6 (2011): 694-702. https://hrcak.srce.hr/78199
Harvard
Žigon, P., et al. (2011). 'Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera', Croatian Medical Journal, 52(6), str. 694-702. Preuzeto s: https://hrcak.srce.hr/78199 (Datum pristupa: 16.06.2021.)
Vancouver
Žigon P, Lakota K, Čučnik S, Švec T, Ambrožič A, Sodin-Šemrl S i sur. Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera. Croat Med J. [Internet]. 2011 [pristupljeno 16.06.2021.];52(6):694-702. Dostupno na: https://hrcak.srce.hr/78199
IEEE
P. Žigon, et al., "Comparison and evaluation of different methodologies and tests for detection of antidsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera", Croatian Medical Journal, vol.52, br. 6, str. 694-702, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/78199. [Citirano: 16.06.2021.]

Sažetak
Aim To evaluate four different commercially available assays
for anti-double stranded DNA (dsDNA) detection and
compare them with the in-house radioimmunoassay according
to Farr (FARR-RIA) in order to select the optimal primary
method for use in combination with FARR-RIA.
Methods Sera from 583 consecutive patients sent to our
laboratory for routine diagnosis, 156 selected patients with
autoimmune diseases (76 systemic lupus erythematosus
[SLE] patients and 80 patients with other autoimmune diseases),
and 150 blood donors were tested for anti-dsDNA
antibodies with two enzyme-linked immunoassays (ELISA),
two Crithidia luciliae immunoflourescence tests (CLIFT),
and FARR-RIA. The specificities and sensitivities of the tests
were calculated and compared.
Results FARR-RIA and CLIFT 2 showed the highest specificity
for SLE (100%), with CLIFT 2 showing higher sensitivity
(33% vs 47%). Both ELISAs showed higher sensitivities
(>53%) than FARR-RIA but lower specificities (<93%),
whereas CLIFT 1 showed the lowest overall agreement
with FARR-RIA.
Conclusion CLIFT 2 was selected as the primary test for
use in combination with FARR-RIA. The use of CLIFT 2 reduced
the number of sera that needed to be tested by
FARR-RIA, the time needed to report the results, and environmental
toxicity, cancerogenicity, and radioactivity.

Ključne riječi
anti-dsDNA antibodies; FARR assay; Crithidia luciliae; systemic lupus erythematosus, enzyme immunoassay; IIF

Hrčak ID: 78199

URI
https://hrcak.srce.hr/78199

Posjeta: 972 *