Izvorni znanstveni članak
Unreliability of a commercial ELISA kit for serologic monitoring of avian influenza
Vladimir Savić
Mirta Balenović
Sažetak
Three-hundred-and-forty-two serum samples from backyard chickens as well as 184 serum samples from farm turkeys, taken in the period from December 27th 2004 to September 9th 2005, were tested using commercial ELISA kit for avian influenza antibodies. It was found 45.6% positive chicken sera and only 0.5% positive turkey sera. Sera that were suspicious or positive in the ELISA were subjected to AGP test for avian influenza antibodies. All samples gave negative result when used AGP test. Specificity assessment of the commercial ELISA kit gave positive results even with monovalent standard antisera for Newcastle disease and CELO virus, whereas antisera for PMV-2 and PMV-3 gave suspicious result. Therefore, the commercial kit should be considered as unreliable for serologic monitoring of avian influenza in poultry. In regards to prevalence of highly pathogenic avian influenza in the world as well as animal and human health hazard posed by H5N1 highly pathogenic avian influenza virus, use of a commercial kit without confirmation with standard serological methods may lead to wrong conclusions with enormous consequences.
Ključne riječi
Avian influenza; Monitoring; ELISA
Hrčak ID:
7745
URI
Datum izdavanja:
25.11.2006.
Posjeta: 1.283 *