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Izvorni znanstveni članak
https://doi.org/10.5562/cca1902
Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time
Stefan Mohr
; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Humboldtstrasse 46, A-8010 Graz, Austria
Goran Sepic
; Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovacica 1, HR-10000 Zagreb, Croatia
Martin Gerhard Schmid
; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Humboldtstrasse 46, A-8010 Graz, Austria
| Puni tekst: engleski, pdf (1 MB) | str. 33-36 | citiraj | |
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APA 6th Edition Mohr, S., Sepic, G. i Schmid, M.G. (2012). Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time. Croatica Chemica Acta, 85 (1), 33-36. https://doi.org/10.5562/cca1902 MLA 8th Edition Mohr, Stefan, et al. "Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time." Croatica Chemica Acta, vol. 85, br. 1, 2012, str. 33-36. https://doi.org/10.5562/cca1902. Citirano 26.02.2021. Chicago 17th Edition Mohr, Stefan, Goran Sepic i Martin Gerhard Schmid. "Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time." Croatica Chemica Acta 85, br. 1 (2012): 33-36. https://doi.org/10.5562/cca1902 Harvard Mohr, S., Sepic, G., i Schmid, M.G. (2012). 'Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time', Croatica Chemica Acta, 85(1), str. 33-36. https://doi.org/10.5562/cca1902 Vancouver Mohr S, Sepic G, Schmid MG. Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time. Croatica Chemica Acta [Internet]. 2012 [pristupljeno 26.02.2021.];85(1):33-36. https://doi.org/10.5562/cca1902 IEEE S. Mohr, G. Sepic i M.G. Schmid, "Chiral Separation of α-hydroxy Acids by Capillary Electrochromatography Using the Cathodic Detergent CTAB as Mobile Phase Additive to Speed up Analysis Time", Croatica Chemica Acta, vol.85, br. 1, str. 33-36, 2012. [Online]. https://doi.org/10.5562/cca1902 |
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Sažetak
In order to speed up the analysis time, working with negatively charged compounds in capillary electromigration techniques often requires to change direction of the EOF. Negatively charged compounds intend to migrate towards the anode and are normally retained by the counter migrating EOF. Therefore the positively charged detergent cetyltrimethylammoniumbromide (CTAB) was used as mobile phase additive for the chiral separation of α-hydroxy acids in capillary electrochromatography (CEC). A 25 % particle loaded Ristocetin A continuous bed (CB) was used as stationary phase to separate a set of mandelic acid derivatives. Using mandelic acid and 4-bromo-mandelic acid as model substances the effect of different CTAB concentrations in the mobile phase on the migration time and the resolution was tested. It could be shown that with an increasing concentration of the CTAB the migration time decreased. The addition of CTAB to the mobile phase is a serious alternative to copolymerizing an anionic charge providing substances such as diallyl dimethylammonium chloride (DADMAC) in CB technology. (doi: 10.5562/cca1902)
Ključne riječi
capillary electrochromatography; CEC, alpha-Hydroxy acids; continuous bed; macrocyclic antibiotics
Hrčak ID: 80410
URI
https://hrcak.srce.hr/80410
Moj profil
Srce