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https://doi.org/10.5562/cca1810

An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect

Tatjana Bačun ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croa
Tatjana Belovari ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia
Aleksandar Včev ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croa
Ivan Mihaljević ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Clinical Institute of Nuclear Medicine and Radiation Protecti
Toni Hanich ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia
Vladimir Fijačko ; J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croatia
Ljubica Glavaš-Obrovac ; J. J. Strossmayer University of Osijek, School of Medicine, University Hospital Centre Osijek, Huttlerova 4, HR-31000 Osijek, Croatia;J. J. Strossmayer University of Osijek, School of Medicine, Internal Medicine Clinic, Huttlerova 4, HR-31000 Osijek, Croa

Puni tekst: engleski, pdf (2 MB) str. 185-191 preuzimanja: 555* citiraj
APA 6th Edition
Bačun, T., Belovari, T., Včev, A., Mihaljević, I., Hanich, T., Fijačko, V. i Glavaš-Obrovac, Lj. (2012). An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect. Croatica Chemica Acta, 85 (2), 185-191. https://doi.org/10.5562/cca1810
MLA 8th Edition
Bačun, Tatjana, et al. "An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect." Croatica Chemica Acta, vol. 85, br. 2, 2012, str. 185-191. https://doi.org/10.5562/cca1810. Citirano 25.01.2020.
Chicago 17th Edition
Bačun, Tatjana, Tatjana Belovari, Aleksandar Včev, Ivan Mihaljević, Toni Hanich, Vladimir Fijačko i Ljubica Glavaš-Obrovac. "An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect." Croatica Chemica Acta 85, br. 2 (2012): 185-191. https://doi.org/10.5562/cca1810
Harvard
Bačun, T., et al. (2012). 'An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect', Croatica Chemica Acta, 85(2), str. 185-191. https://doi.org/10.5562/cca1810
Vancouver
Bačun T, Belovari T, Včev A, Mihaljević I, Hanich T, Fijačko V i sur. An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect. Croatica Chemica Acta [Internet]. 2012 [pristupljeno 25.01.2020.];85(2):185-191. https://doi.org/10.5562/cca1810
IEEE
T. Bačun, et al., "An Overexpression of Icam-1 in Mild Hyperhomocysteinemia and Hyperglycemia − A Study of Antidiabetics Administration Effect", Croatica Chemica Acta, vol.85, br. 2, str. 185-191, 2012. [Online]. https://doi.org/10.5562/cca1810

Sažetak
Elevated plasma homocysteine is connected to atherosclerosis and increased cerebrovascular
and ischemic heart disease especially in the patients with type 2 diabetes. Effects of metformin (10 μg
mL−1), insulin (1 mUI mL−1), and their combination administration in condition of mild
hyperhomocysteinemia (30 μmol dm−3) and hyperglycemia (12 mmol dm−3) on human aortic endothelial
cells (HAEC) was investigated. HAEC were cultured 4 h in a medium with homocysteine (7 and 30 μmol
dm−3) and glucose (5.5 and 12.0 mmol dm−3). E-selectin, intercellular adhesion molecule-1 (ICAM-1), and
vascular adhesion molecule-1 (VCAM-1) expressions were analysed by flow-cytometry. Controls were
CAMs expression on HAEC in medium with physiological concentration of homocysteine and glucose.
HAEC, incubated additionally with 30 μmol dm−3 homocysteine, increased expression of ICAM-1 for 65
% (p = 0.07) and decreased of E-selectin for 30 % (p = 0.07) and VCAM-1 for 13 % (0.06). In condition
of mild hyperhomocysteinemia and hyperglycemia there was a statistically significant increase of ICAM-
1 expression (for 22 %, p = 0.04), while administration of metformin and insulin did not statistically significantly
influence adhesion molecules expression. Observed significant overexpression of ICAM-1,
leads us to conclude that combination of mild hyperhomocisteinemia and hyperglycemia could have significant
influence on development of inflammation and atherosclerosis in diabetic patients.(doi:
10.5562/cca1810)

Ključne riječi
Cell adhesion molecules; human aortic endothelial cells; mild hyperhomocysteinemia; mild hyperglycemia; metformin; insulin

Hrčak ID: 84531

URI
https://hrcak.srce.hr/84531

Posjeta: 800 *