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Izvorni znanstveni članak
https://doi.org/10.5562/cca2146
Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA
Vlatka Godinić-Mikulčić
orcid.org/0000-0003-0319-8035
; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
Jelena Jarić
; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ivana Weygand-Đurašević
; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
| Puni tekst: engleski, pdf (6 MB) | str. 441-449 | citiraj | |
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APA 6th Edition Godinić-Mikulčić, V., Jarić, J. i Weygand-Đurašević, I. (2012). Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA. Croatica Chemica Acta, 85 (4), 441-449. https://doi.org/10.5562/cca2146 MLA 8th Edition Godinić-Mikulčić, Vlatka, et al. "Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA." Croatica Chemica Acta, vol. 85, br. 4, 2012, str. 441-449. https://doi.org/10.5562/cca2146. Citirano 04.03.2021. Chicago 17th Edition Godinić-Mikulčić, Vlatka, Jelena Jarić i Ivana Weygand-Đurašević. "Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA." Croatica Chemica Acta 85, br. 4 (2012): 441-449. https://doi.org/10.5562/cca2146 Harvard Godinić-Mikulčić, V., Jarić, J., i Weygand-Đurašević, I. (2012). 'Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA', Croatica Chemica Acta, 85(4), str. 441-449. https://doi.org/10.5562/cca2146 Vancouver Godinić-Mikulčić V, Jarić J, Weygand-Đurašević I. Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA. Croatica Chemica Acta [Internet]. 2012 [pristupljeno 04.03.2021.];85(4):441-449. https://doi.org/10.5562/cca2146 IEEE V. Godinić-Mikulčić, J. Jarić i I. Weygand-Đurašević, "Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA", Croatica Chemica Acta, vol.85, br. 4, str. 441-449, 2012. [Online]. https://doi.org/10.5562/cca2146 |
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Sažetak
Several studies have revealed the involvement of multi aminoacyl-tRNA synthetase complexes (MSC) in archaeal and eukaryotic translation. Here we analyzed interactions of atypical Methanothermobacter thermautotrophicus seryl-tRNA synthetase (MtSerRS), transfer RNA (tRNASer) and arginyl-tRNA synthetase (ArgRS). Surface plasmon resonance (SPR) was used to determine dissociation constants for the MtSerRS:tRNASer complex and the results were consistent with cooperative binding of tRNASer. This finding was supported by the ability of MtSerRS to bind two tRNAs in gel mobility shift assay. Notably, the MtSerRS:tRNASer complex formation was stimulated by MtArgRS, previously determined interacting partner of MtSerRS. MtArgRS decreases Kd for MtSerRS:tRNASer two-fold, but does not affect cooperative properties or stoichiometry of the complex. Further investigation of complex formation between MtSerRS and tRNASer showed that this molecular interaction is salt-dependent. The most pronounced improvements in binding were determined at high ionic strength, using Tris as a buffering agent, while the addition of Mg2+ ions led to the same SPR response. (doi: 10.5562/cca2146)
Ključne riječi
aminoacyl-tRNA synthetase; seryl-tRNA synthetase; arginyl-tRNA synthetase; tRNA; surface plasmon resonance
Hrčak ID: 93732
URI
https://hrcak.srce.hr/93732
Moj profil
Srce