hrcak mascot   Srce   HID

Izvorni znanstveni članak

Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions

Ita Gruić-Sovulj ; Department of Chemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia
Jasmina Rokov-Plavec ; Department of Chemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia
Marko Močibob ; Department of Chemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia
Tomislav Kamenski ; Department of Chemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia
Ivana Weygand-Đurašević ; Department of Chemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia

Puni tekst: engleski, pdf (125 KB) str. 599-604 preuzimanja: 215* citiraj
APA 6th Edition
Gruić-Sovulj, I., Rokov-Plavec, J., Močibob, M., Kamenski, T. i Weygand-Đurašević, I. (2004). Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions. Croatica Chemica Acta, 77 (4), 599-604. Preuzeto s https://hrcak.srce.hr/102984
MLA 8th Edition
Gruić-Sovulj, Ita, et al. "Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions." Croatica Chemica Acta, vol. 77, br. 4, 2004, str. 599-604. https://hrcak.srce.hr/102984. Citirano 29.09.2020.
Chicago 17th Edition
Gruić-Sovulj, Ita, Jasmina Rokov-Plavec, Marko Močibob, Tomislav Kamenski i Ivana Weygand-Đurašević. "Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions." Croatica Chemica Acta 77, br. 4 (2004): 599-604. https://hrcak.srce.hr/102984
Harvard
Gruić-Sovulj, I., et al. (2004). 'Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions', Croatica Chemica Acta, 77(4), str. 599-604. Preuzeto s: https://hrcak.srce.hr/102984 (Datum pristupa: 29.09.2020.)
Vancouver
Gruić-Sovulj I, Rokov-Plavec J, Močibob M, Kamenski T, Weygand-Đurašević I. Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions. Croatica Chemica Acta [Internet]. 2004 [pristupljeno 29.09.2020.];77(4):599-604. Dostupno na: https://hrcak.srce.hr/102984
IEEE
I. Gruić-Sovulj, J. Rokov-Plavec, M. Močibob, T. Kamenski i I. Weygand-Đurašević, "Stability of the Complex between Yeast Seryl-tRNA Synthetase and tRNASer under Different Electrophoretic Conditions", Croatica Chemica Acta, vol.77, br. 4, str. 599-604, 2004. [Online]. Dostupno na: https://hrcak.srce.hr/102984. [Citirano: 29.09.2020.]

Sažetak
Noncovalent interactions of yeast homodimeric seryl-tRNA synthetase (SerRS) and cognate tRNASer were studied by the gel mobility shift assay and zone-interference gel electrophoresis performed under the same binding and electrophoretic conditions. Purified tRNASer as well as total yeast tRNA were applied as ligands. In the absence of Mg2+, SerRS:(tRNASer)1 noncovalent complex was detected only by zone-interference gel electrophoresis. Kd values determined in the presence and absence of Mg2+ were in the same range, suggesting that Mg2+ ions mainly influence dissociation-association kinetics of the complex, with a minor contribution to its thermodynamic stability. Comparison of these two assays was shown to be useful in the analysis of thermodynamic and kinetic properties of protein:nucleic acid complexes.

Ključne riječi
aminoacyl-tRNA synthetase; gel mobility shift assay; zone-interference gel electrophoresis; SerRS:tRNASer noncovalent complexes; Mg2+ influence

Hrčak ID: 102984

URI
https://hrcak.srce.hr/102984

Posjeta: 346 *