Izvorni znanstveni članak
Release of β-galactosidase by Permeabilization of Indigenously Isolated Lactobacillus acidophilus Using Lysozyme
H. S. Choonia
; Food Engineering and Technology Department, Institute of Chemical Technology (Deemed University) formerly UDCT, Matunga, Mumbai – 400 019, India
S. S. Lele
; Food Engineering and Technology Department, Institute of Chemical Technology (Deemed University) formerly UDCT, Matunga, Mumbai – 400 019, India
Sažetak
Enzymatic lysis, using lysozyme, has been shown in this paper to be an effective cell lysis method to release β-galactosidase from Lactobacillus acidophilus indigenously isolated from Eleusine coracana. The lytic process was developed using response surface
methodology (RSM) to optimize lysozyme concentration, cell density and incubation time critical for the release of β-galactosidase. The optimized permeabilization condition (lysozyme: 33.63 · 104 U mL–1; cell density: 4.7 % w/v on wet basis; incubation time: 10h 30min.) resulted in 1.2 fold increase in release of β-galactosidase when compared with an optimized ultrasonication process. These optimized conditions were used to determine the release constants of β-galactosidase and total protein release as a function of temperature. The enzyme and protein release constants were used further to calculate the location factor of β-galactosidase.
Ključne riječi
Lactobacillus acidophilus; β-galactosidase; enzymatic permeabilization; response surface methodology; location factor; lysozyme
Hrčak ID:
112355
URI
Datum izdavanja:
19.12.2013.
Posjeta: 923 *