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Acta Pharmaceutica, Vol. 64 No. 3, 2014.

Izvorni znanstveni članak
https://doi.org/10.2478/acph-2014-0025

Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis

MOHAMMED AUWAL IBRAHIM ; School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, 4000 South Africa; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
NEIL ANTHONY KOORBANALLY ; School of Chemistry and Physics, University of KwaZulu-Natal (Westville Campus), Durban, 4000 South Africa
MD. SHAHIDUL ISLAM ; School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, 4000 South Africa

Puni tekst: engleski, pdf (631 KB) str. 311-324 preuzimanja: 642* citiraj
APA 6th Edition
IBRAHIM, M.A., KOORBANALLY, N.A. i ISLAM, M.S. (2014). Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis. Acta Pharmaceutica, 64 (3), 311-324. https://doi.org/10.2478/acph-2014-0025
MLA 8th Edition
IBRAHIM, MOHAMMED AUWAL, et al. "Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis." Acta Pharmaceutica, vol. 64, br. 3, 2014, str. 311-324. https://doi.org/10.2478/acph-2014-0025. Citirano 20.02.2019.
Chicago 17th Edition
IBRAHIM, MOHAMMED AUWAL, NEIL ANTHONY KOORBANALLY i MD. SHAHIDUL ISLAM. "Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis." Acta Pharmaceutica 64, br. 3 (2014): 311-324. https://doi.org/10.2478/acph-2014-0025
Harvard
IBRAHIM, M.A., KOORBANALLY, N.A., i ISLAM, M.S. (2014). 'Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis', Acta Pharmaceutica, 64(3), str. 311-324. doi: https://doi.org/10.2478/acph-2014-0025
Vancouver
IBRAHIM MA, KOORBANALLY NA, ISLAM MS. Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis. Acta Pharm. [Internet]. 2014 [pristupljeno 20.02.2019.];64(3):311-324. doi: https://doi.org/10.2478/acph-2014-0025
IEEE
M.A. IBRAHIM, N.A. KOORBANALLY i M.S. ISLAM, "Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes(α-glucosidase and α-amylase) by Khaya senegalensis", Acta Pharmaceutica, vol.64, br. 3, str. 311-324, 2014. [Online]. doi: https://doi.org/10.2478/acph-2014-0025

Sažetak
This study evaluated the in vitro anti-oxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and then tested for anti-oxidative activity. Our findings revealed that the ethanolic extract of the root had the highest anti-oxidative activity. Solvent-solvent fractionation of the root ethanolic extract yielded a butanol fraction that showed higher anti-oxidative activity than other fractions. Furthermore, the butanol fraction had significantly higher (p < 0.05) α-glucosidase and α-amylase inhibitory activities with IC50 values of 2.89 ± 0.46 and 97.51 ± 5.72 μg mL–1, respectively. Enzyme kinetic studies indicated that the butanol fraction is a non-competitive inhibitor for α-glucosidase with an inhibition binding constant Ki of 1.30 μg mL–1 and a competitive inhibitor of α-amylase with a Ki of 7.50 μg mL–1. GC-MS analysis revealed that the butanol fraction contained two chromones, p-anilinophenol and 3-ethyl-5-(3-ethyl-(3H)-benzothiazol-2-ylidene)-2-(p-tolylvinylamino)-4-thiazolidinone.Data obtained in the study suggest that the butanol fraction derived from the ethanolic extract of K. senegalensis root possessed excellent anti-oxidative as well as α-glucosidase and α-amylase inhibitory activities while chromones and/or p-anilinophenol could be the main bioactive compounds responsible for the observed activities.

Ključne riječi
Khaya senegalensis (Meliaceae); type-2 diabetes; α-glucosidase; α-amylase; oxidative stress

Hrčak ID: 121069

URI
https://hrcak.srce.hr/121069

Posjeta: 741 *