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Veterinarski arhiv, Vol. 84 No. 3, 2014.

Izvorni znanstveni članak

Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.

Bharat B. Bhanderi ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Mayurdhvaj K. Jhala ; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Viral B. Ahir ; Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Vaibhav D. Bhatt ; Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Chaitanya G. Joshi ; Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

Puni tekst: engleski, pdf (198 KB) str. 215-228 preuzimanja: 763* citiraj
APA 6th Edition
Bhanderi, B.B., Jhala, M.K., Ahir, V.B., Bhatt, V.D. i Joshi, C.G. (2014). Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.. Veterinarski arhiv, 84 (3), 215-228. Preuzeto s https://hrcak.srce.hr/121943
MLA 8th Edition
Bhanderi, Bharat B., et al. "Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.." Veterinarski arhiv, vol. 84, br. 3, 2014, str. 215-228. https://hrcak.srce.hr/121943. Citirano 24.04.2019.
Chicago 17th Edition
Bhanderi, Bharat B., Mayurdhvaj K. Jhala, Viral B. Ahir, Vaibhav D. Bhatt i Chaitanya G. Joshi. "Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.." Veterinarski arhiv 84, br. 3 (2014): 215-228. https://hrcak.srce.hr/121943
Harvard
Bhanderi, B.B., et al. (2014). 'Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.', Veterinarski arhiv, 84(3), str. 215-228. Preuzeto s: https://hrcak.srce.hr/121943 (Datum pristupa: 24.04.2019.)
Vancouver
Bhanderi BB, Jhala MK, Ahir VB, Bhatt VD, Joshi CG. Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.. Veterinarski arhiv [Internet]. 2014 [pristupljeno 24.04.2019.];84(3):215-228. Dostupno na: https://hrcak.srce.hr/121943
IEEE
B.B. Bhanderi, M.K. Jhala, V.B. Ahir, V.D. Bhatt i C.G. Joshi, "Cultural and metagenomic based identification of a microbiome from subclinical mastitis in cows.", Veterinarski arhiv, vol.84, br. 3, str. 215-228, 2014. [Online]. Dostupno na: https://hrcak.srce.hr/121943. [Citirano: 24.04.2019.]

Sažetak
Metagenomic and traditional microbial culture based analyses of milk samples from cows harbouring subclinical mastitis pathogens were carried out to identify the microbial community structure of milk. A total of 77 Triple cross (TP), Kankrej and Gir lactating cows and 301 quarters were screened for subclinical mastitis. A total of 106 isolates belonging to five different microbial genera were recovered from 91 quarters of 41 cows, including 15 quarters having mixed bacterial infections by cultural examination. Pyrosequencing readings obtained from the breed wise pooled DNA of subclinical mastitis milk samples were analyzed using the SEED subsystem database of Meta Genome Rapid Annotation with Subsystem Technology (MG-RAST). Among the five genera, Staphylococcus, Streptococcus, Micrococcus, Bacillus and Escherichia, detected in the subclinical mastitis milk samples by culture based methods, four genera, Staphylococcus, Streptococcus, Bacillus and Escherichia, were identified in the corresponding pyrosequencing data, while Micrococcus was not found. In contrast, the pyrosequencing yielded 28 bacterial species, of which only two species, S. aureus and E. coli, were identified by the cultural method. S. agalactiae, the third species identified by cultural method, was not found in the pyrosequencing data. Metagenomic analysis additionally identified 19 genera and 26 species in comparison with the routine cultural methods. Many of the fastidious / anaerobic bacterial organisms, which are difficult to cultivate by routine methods, were identified by metagenomic analyses.

Ključne riječi
subclinical mastitis; metagenomic; cows

Hrčak ID: 121943

URI
https://hrcak.srce.hr/121943

[hrvatski]

Posjeta: 923 *