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Kratko priopćenje
https://doi.org/10.2478/acph-2014-0031

TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound

DAMJAN JANEŠ ; Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia
SAMO KREFT ; Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia

Puni tekst: engleski, pdf (513 KB) str. 503-508 preuzimanja: 389* citiraj
APA 6th Edition
JANEŠ, D. i KREFT, S. (2014). TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound. Acta Pharmaceutica, 64 (4), 503-508. https://doi.org/10.2478/acph-2014-0031
MLA 8th Edition
JANEŠ, DAMJAN i SAMO KREFT. "TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound." Acta Pharmaceutica, vol. 64, br. 4, 2014, str. 503-508. https://doi.org/10.2478/acph-2014-0031. Citirano 17.06.2019.
Chicago 17th Edition
JANEŠ, DAMJAN i SAMO KREFT. "TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound." Acta Pharmaceutica 64, br. 4 (2014): 503-508. https://doi.org/10.2478/acph-2014-0031
Harvard
JANEŠ, D., i KREFT, S. (2014). 'TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound', Acta Pharmaceutica, 64(4), str. 503-508. doi: https://doi.org/10.2478/acph-2014-0031
Vancouver
JANEŠ D, KREFT S. TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound. Acta Pharm. [Internet]. 2014 [pristupljeno 17.06.2019.];64(4):503-508. doi: https://doi.org/10.2478/acph-2014-0031
IEEE
D. JANEŠ i S. KREFT, "TLC densitometric method for analysis of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound", Acta Pharmaceutica, vol.64, br. 4, str. 503-508, 2014. [Online]. doi: https://doi.org/10.2478/acph-2014-0031

Sažetak
Due to severe toxicity of pyrrolizidine alkaloids, their quantification in medicinal products is very important. The idea of this research was to use retrorsine as a surrogate reference compound instead of lycopsamine reference or lycopsamine isolated from comfrey. A method for the analysis of licopsamine in extracts of comfrey roots was developed and validated, employing thin layer chromatography, derivatisation with Dann-Mattocks reagent followed by densitometric analysis. The new method showed linearity within 0.70 to 7.0 μg of lycopsamine per application of 10 μL of a solution on a plate. It has also been proven to be specific and precise (repeatability RSD 2–4 % within the plate). The method was successfully employed for quantification of lycopsamine in comfrey root and comfrey root medicinal products such as ointments.

Ključne riječi
comfrey; lycopsamine; retrorsine; TLC; densitometry

Hrčak ID: 123132

URI
https://hrcak.srce.hr/123132

Posjeta: 534 *