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Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-65-2014-2494

Jedinstveni obrazac djelovanja bisfenola A na ekspresiju gena čimbenika rasta živca embrionske mišje stanične linije N-44 dobivene iz hipotalamusa

Katsuhiko Warita ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Tomoko Mitsuhashi ; Department of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, USA
Nobuhiko Hoshi ; Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
Ken-ichi Ohta ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Shingo Suzuki ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Yoshiki Takeuchi ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Takanori Miki ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan

Puni tekst: engleski, pdf (343 KB) str. 293-298 preuzimanja: 476* citiraj
APA 6th Edition
Warita, K., Mitsuhashi, T., Hoshi, N., Ohta, K., Suzuki, S., Takeuchi, Y. i Miki, T. (2014). A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44. Arhiv za higijenu rada i toksikologiju, 65 (3), 293-298. https://doi.org/10.2478/10004-1254-65-2014-2494
MLA 8th Edition
Warita, Katsuhiko, et al. "A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44." Arhiv za higijenu rada i toksikologiju, vol. 65, br. 3, 2014, str. 293-298. https://doi.org/10.2478/10004-1254-65-2014-2494. Citirano 24.02.2021.
Chicago 17th Edition
Warita, Katsuhiko, Tomoko Mitsuhashi, Nobuhiko Hoshi, Ken-ichi Ohta, Shingo Suzuki, Yoshiki Takeuchi i Takanori Miki. "A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44." Arhiv za higijenu rada i toksikologiju 65, br. 3 (2014): 293-298. https://doi.org/10.2478/10004-1254-65-2014-2494
Harvard
Warita, K., et al. (2014). 'A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44', Arhiv za higijenu rada i toksikologiju, 65(3), str. 293-298. https://doi.org/10.2478/10004-1254-65-2014-2494
Vancouver
Warita K, Mitsuhashi T, Hoshi N, Ohta K, Suzuki S, Takeuchi Y i sur. A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44. Arh Hig Rada Toksikol. [Internet]. 2014 [pristupljeno 24.02.2021.];65(3):293-298. https://doi.org/10.2478/10004-1254-65-2014-2494
IEEE
K. Warita, et al., "A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44", Arhiv za higijenu rada i toksikologiju, vol.65, br. 3, str. 293-298, 2014. [Online]. https://doi.org/10.2478/10004-1254-65-2014-2494

Sažetak
U istraživanju toksičnosti bisfenola A (BPA) utvrđena je ekspresija gena čimbenika rasta živca (eng. nerve growth factor - NGF) embrionske mišje stanične linije mHypoE-N44 dobivene iz hipotalamusa nakon trosatnog izlaganja BPA-u u rasponu doza od 0,02 do 200 μmol L-1. Razine Ngf mRNA snizile su se ovisno o dozi, a značajne razlike od kontrolne skupine zamijećene su za raspon od 2 do 50 μmol L-1. Međutim, počevši od doze od 100 do 200 μmol L-1, razine Ngf mRNA značajno su se povećale u odnosu na kontrolu, a ekspresija gena kaspaze 3 i transformacijskog proteina 73 značajno snizila. Ti rezultati upućuju na to da visoke doze BPA u embrionskoj hipotalamičkoj staničnoj liniji stvaraju jedinstveni obrazac ekspresije gena Ngf te da BPA može suprimirati apoptozu koja je nužna za rani razvoj mozga.

Ključne riječi
čimbenik rasta živca; fetalni hipotalamus; kaspaza 3; razvojna toksičnost; transformacijski protein 73

Hrčak ID: 126612

URI
https://hrcak.srce.hr/126612

[engleski]

Posjeta: 797 *