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Original scientific paper

Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats

Nikola Radović ; University of Zagreb, University Hospital Dubrava, Department of Urology, Zagreb, Croatia
Gorana Aralica ; University of Zagreb, School of Medicine, University Hospital Dubrava Department of Pathology, Institute of Pathology, Zagreb, Croatia
Danica Galesić Ljubanović ; University of Zagreb, School of Medicine, University Hospital Dubrava Department of Pathology, Institute of Pathology, Zagreb, Croatia
Vjekoslav Jelec ; University of Zagreb, University Hospital Dubrava, Department of Neurosurgery, Zagreb, Croatia
Mladen Kontek ; University of Zagreb, University Hospital Merkur, Department of Nephrology, Zagreb, Croatia

Fulltext: english, pdf (229 KB) pages 583-588 downloads: 316* cite
APA 6th Edition
Radović, N., Aralica, G., Galesić Ljubanović, D., Jelec, V. & Kontek, M. (2014). Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats. Collegium antropologicum, 38 (2), 583-588. Retrieved from https://hrcak.srce.hr/127565
MLA 8th Edition
Radović, Nikola, et al. "Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats." Collegium antropologicum, vol. 38, no. 2, 2014, pp. 583-588. https://hrcak.srce.hr/127565. Accessed 4 Apr. 2020.
Chicago 17th Edition
Radović, Nikola, Gorana Aralica, Danica Galesić Ljubanović, Vjekoslav Jelec and Mladen Kontek. "Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats." Collegium antropologicum 38, no. 2 (2014): 583-588. https://hrcak.srce.hr/127565
Harvard
Radović, N., et al. (2014). 'Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats', Collegium antropologicum, 38(2), pp. 583-588. Available at: https://hrcak.srce.hr/127565 (Accessed 04 April 2020)
Vancouver
Radović N, Aralica G, Galesić Ljubanović D, Jelec V, Kontek M. Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats. Collegium antropologicum [Internet]. 2014 [cited 2020 April 04];38(2):583-588. Available from: https://hrcak.srce.hr/127565
IEEE
N. Radović, G. Aralica, D. Galesić Ljubanović, V. Jelec and M. Kontek, "Effect of Unilateral Ureteral Obstruction and Anti-Angiotensin II Treatment on Renal Tubule Cell Apoptosis and Interstitial Fibrosis in Rats", Collegium antropologicum, vol.38, no. 2, pp. 583-588, 2014. [Online]. Available: https://hrcak.srce.hr/127565. [Accessed: 04 April 2020]

Abstracts
Unilateral ureteral obstruction (UUO) results in a number of pathophysiological and morphological changes in the renal parenchyma, including interstitial inflammation and fibrosis, apoptotic changes of tubular and interstitial cells. Recent studies have indicated an association between renin-angiotensin system and apoptotic alterations in the kidney after unilateral obstructive nephropathy. In this study, the effect of ACE inhibitors and AT1 receptor antagonists on tubular cell apoptosis and interstitial fibrosis in obstructive nephropathy after UUO in rats was investigated. The study was conducted on Wistar rats with unilaterally ligated ureter and sham operated animals (control group). The rats with UUO were treated with ACE inhibitor (cilazapril) or AT1 receptor antagonists (losartan) and control group was treated with H2O. Sham-operated animals were treated in the same way. Tubular and interstitial cell apoptosis was detected morphologically by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL). The area of intersitial fibrosis was determined using computer-assisted image processing after Gomory silver impregnation of paraffin sections. All experimental animal groups with unilateral ureter ligation showed a significantly increased number of apoptotic tubular and interstitial cells in the obstructed kidney compared with the contralateral, unobstructed kidney. Histomorphometric analysis of renal interstitial fibrotic changes in the groups of rats treated with losartan or water showed a statistically significant difference (p < 0.05) between the operated and sham-operated animals. In conclusion, following UUO there is a significantly increased number of apoptotic tubular cells and interstitial fibrosis in the ipsilateral kidney compared with the contralateral kidney. ACE inhibitors and AT1 receptor antagonists could not decrease the extent of renal cells apoptosis and interstitial fibrosis after UUO.

Keywords
obstructive nephropathy; apoptosis; angiotensin II; interstial fibrosis; ACE inhibitors; AT1 receptor antagonist

Hrčak ID: 127565

URI
https://hrcak.srce.hr/127565

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