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Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae

Ita Gruić Sovulj ; Laboratory of Biochemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia
Ivana Weygand-Đurašević ; Laboratory of Biochemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia
Željko Kućan ; Laboratory of Biochemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia

Puni tekst: engleski, pdf (154 KB) str. 161-171 preuzimanja: 118* citiraj
APA 6th Edition
Gruić Sovulj, I., Weygand-Đurašević, I. i Kućan, Ž. (2001). Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae. Croatica Chemica Acta, 74 (1), 161-171. Preuzeto s https://hrcak.srce.hr/131785
MLA 8th Edition
Gruić Sovulj, Ita, et al. "Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae." Croatica Chemica Acta, vol. 74, br. 1, 2001, str. 161-171. https://hrcak.srce.hr/131785. Citirano 06.03.2021.
Chicago 17th Edition
Gruić Sovulj, Ita, Ivana Weygand-Đurašević i Željko Kućan. "Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae." Croatica Chemica Acta 74, br. 1 (2001): 161-171. https://hrcak.srce.hr/131785
Harvard
Gruić Sovulj, I., Weygand-Đurašević, I., i Kućan, Ž. (2001). 'Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae', Croatica Chemica Acta, 74(1), str. 161-171. Preuzeto s: https://hrcak.srce.hr/131785 (Datum pristupa: 06.03.2021.)
Vancouver
Gruić Sovulj I, Weygand-Đurašević I, Kućan Ž. Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae. Croatica Chemica Acta [Internet]. 2001 [pristupljeno 06.03.2021.];74(1):161-171. Dostupno na: https://hrcak.srce.hr/131785
IEEE
I. Gruić Sovulj, I. Weygand-Đurašević i Ž. Kućan, "Influence of Modified tRNATyr on the Activation of Tyrosine Catalyzed by Tyrosyl-tRNA Synthetase from Saccharomyces cerevisiae", Croatica Chemica Acta, vol.74, br. 1, str. 161-171, 2001. [Online]. Dostupno na: https://hrcak.srce.hr/131785. [Citirano: 06.03.2021.]

Sažetak
Yeast tyrosyl-tRNA synthetase (TyrRS, EC 6.1.1.1) is a homodimeric enzyme capable of binding only one molecule of its macromolecular substrate, tRNATyr. The reactive intermediate tyrosyl adenylate is formed from tyrosine and ATP in the first reaction step, which can be conveniently assayed by pyrophosphate exchange. In order to determine the number of active sites per homodimer, the kinetics of pyrophosphate exchange was measured in the presence of the tRNATyr analogue unable to accept the amino acid. The analogue was found to form the expected equimolar complex with dimeric enzyme. It was a competitive inhibitor of pyrophosphate exchange with respect to ATP and non-competitive with respect to tyrosine. Inhibition cannot exceed 50%, suggesting the simplest model in which yeast TyrRS is a symmetrical dimer, possessing two identical active sites, both capable of catalyzing the formation of tyrosyl adenylate.

Ključne riječi
modified tRNATyr; pyrophosphate exchange; Saccharo-myces cerevisiae; tyrosyl-tRNA synthetase; tRNATyr

Hrčak ID: 131785

URI
https://hrcak.srce.hr/131785

Posjeta: 227 *