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Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP

Stjepan Orhanović   ORCID icon orcid.org/0000-0001-8327-037X ; University of Split, Faculty of Natural Sciences, Mathematics and Education, Department of Chemistry, N. Tesle 12, 21000 Split, Croatia
Maja Pavela-Vrančič ; University of Split, Faculty of Natural Sciences, Mathematics and Education, Department of Chemistry, N. Tesle 12, 21000 Split, Croatia

Puni tekst: engleski, pdf (92 KB) str. 819-830 preuzimanja: 601* citiraj
APA 6th Edition
Orhanović, S. i Pavela-Vrančič, M. (2000). Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP. Croatica Chemica Acta, 73 (3), 819-830. Preuzeto s https://hrcak.srce.hr/132020
MLA 8th Edition
Orhanović, Stjepan i Maja Pavela-Vrančič. "Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP." Croatica Chemica Acta, vol. 73, br. 3, 2000, str. 819-830. https://hrcak.srce.hr/132020. Citirano 21.09.2020.
Chicago 17th Edition
Orhanović, Stjepan i Maja Pavela-Vrančič. "Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP." Croatica Chemica Acta 73, br. 3 (2000): 819-830. https://hrcak.srce.hr/132020
Harvard
Orhanović, S., i Pavela-Vrančič, M. (2000). 'Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP', Croatica Chemica Acta, 73(3), str. 819-830. Preuzeto s: https://hrcak.srce.hr/132020 (Datum pristupa: 21.09.2020.)
Vancouver
Orhanović S, Pavela-Vrančič M. Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP. Croatica Chemica Acta [Internet]. 2000 [pristupljeno 21.09.2020.];73(3):819-830. Dostupno na: https://hrcak.srce.hr/132020
IEEE
S. Orhanović i M. Pavela-Vrančič, "Alkaline Phosphatase Activity in Seawater: Influence of Reaction Conditions on the Kinetic Parameters of ALP", Croatica Chemica Acta, vol.73, br. 3, str. 819-830, 2000. [Online]. Dostupno na: https://hrcak.srce.hr/132020. [Citirano: 21.09.2020.]

Sažetak
Alkaline phosphatase (ALP) is an extracellular enzyme enabling utilisation of phosphomonoesters as the source of inorganic phosphate (Pi) required for the maintenance of cellular metabolism. Its catalytic properties have been extensively studied in aquatic environments in an attempt to describe its influence on phosphorous cycling in natural waters. Despite its significance and frequent determination, no standard method has been developed so far. In order to examine the effect of various reaction conditions on the kinetic properties of ALP in seawater, the stability of ALP in chloroform-stabilised samples has been studied in addition to the influence of the buffer type, its concentration and pH, the metal-ion content, Pi and incubation time. It might be concluded that the kinetic properties of ALP are significantly influenced by the buffer pH, the concentration of Mg2+ and Zn2+ ions and the incubation period, requiring a precise definition of these reaction parameters in determining a standard method for the measurement of ALP activity in water media.

Ključne riječi
aquatic enzymes; alkaline phosphatase; kinetic properties; method optimisation

Hrčak ID: 132020

URI
https://hrcak.srce.hr/132020

Posjeta: 748 *