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Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A

Nicola S. Hunter ; Section of Structural Chemistry, Department of Chemistry, University of Manchester, M13 9PL, UK
Thomas J. Gleichmann ; Section of Structural Chemistry, Department of Chemistry, University of Manchester, M13 9PL, UK
Madeleine Helliwell ; Section of Structural Chemistry, Department of Chemistry, University of Manchester, M13 9PL, UK
Luisa Barba ; Istituto di Strutturista Chimica, CNR, Stada Statale 14, Km 163.5, Basovizza, 34102 Trieste, Italy
Edoardo Busetto ; Sincrotrone Trieste, Stada Statale 14, Km 163.5, Basovizza, 34102 Trieste, Italy
Andrea Lausi ; Sincrotrone Trieste, Stada Statale 14, Km 163.5, Basovizza, 34102 Trieste, Italy
Helen J. Price ; Section of Structural Chemistry, Department of Chemistry, University of Manchester, M13 9PL, UK
John R. Helliwell ; Section of Structural Chemistry, Department of Chemistry, University of Manchester, M13 9PL, UK

Puni tekst: engleski, pdf (201 KB) str. 673-684 preuzimanja: 209* citiraj
APA 6th Edition
Hunter, N.S., Gleichmann, T.J., Helliwell, M., Barba, L., Busetto, E., Lausi, A., ... Helliwell, J.R. (1999). Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A. Croatica Chemica Acta, 72 (2-3), 673-684. Preuzeto s https://hrcak.srce.hr/132264
MLA 8th Edition
Hunter, Nicola S., et al. "Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A." Croatica Chemica Acta, vol. 72, br. 2-3, 1999, str. 673-684. https://hrcak.srce.hr/132264. Citirano 06.04.2020.
Chicago 17th Edition
Hunter, Nicola S., Thomas J. Gleichmann, Madeleine Helliwell, Luisa Barba, Edoardo Busetto, Andrea Lausi, Helen J. Price i John R. Helliwell. "Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A." Croatica Chemica Acta 72, br. 2-3 (1999): 673-684. https://hrcak.srce.hr/132264
Harvard
Hunter, N.S., et al. (1999). 'Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A', Croatica Chemica Acta, 72(2-3), str. 673-684. Preuzeto s: https://hrcak.srce.hr/132264 (Datum pristupa: 06.04.2020.)
Vancouver
Hunter NS, Gleichmann TJ, Helliwell M, Barba L, Busetto E, Lausi A i sur. Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A. Croatica Chemica Acta [Internet]. 1999 [pristupljeno 06.04.2020.];72(2-3):673-684. Dostupno na: https://hrcak.srce.hr/132264
IEEE
N.S. Hunter, et al., "Crystallographic MAD Phasing Strategies Explored Using ELETTRA Sincrotrone Mn K-Edge Data to 2.1 Å and Use of CHESS Establishes the Diffraction Resolution Limit as 0.92 Å for the Protein Mn, Ca Concanavalin A", Croatica Chemica Acta, vol.72, br. 2-3, str. 673-684, 1999. [Online]. Dostupno na: https://hrcak.srce.hr/132264. [Citirano: 06.04.2020.]

Sažetak
Multiwavelength anomalous dispersion (MAD) data have been collected from a single crystal of the protein concanavalin A so as to evaluate different combinations of wavelengths for crystallographic structure determination. Data were recorded to 2.1 Å resolution on a flash frozen crystal at three wavelengths about the Mn K-edge (1.8951 Å, 1.8940 Å, 1.800 Å) using synchrotron radiation at ELETTRA's Sincrotrone Trieste 'XRD' beamline. This is one of the longest wavelength K-edge MAD studies undertaken to date. Anomalous and dispersive Patterson maps are seen to be of high quality and indicate a high occupancy for the manganese binding site. This is confirmed also in the MAD phase determination and electron density maps. Finally 0.92 Å data recorded at CHESS indicates the prospects available for combined phasing strategies based on MAD to medium/high resolution along with ultra high resolution data.

Ključne riječi
Mn; Ca concanavalin A; protein crystallography; MAD phasing; ultra-high resolution; ELETTRA sincrotrone; CHESS synchrotron; Mn K-edge; lectin protein; direct methods in protein crystallography

Hrčak ID: 132264

URI
https://hrcak.srce.hr/132264

Posjeta: 325 *