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Croatica Chemica Acta, Vol.80 No.1 Ožujak 2007.

Izvorni znanstveni članak

Preliminary Crystallographic Study of Streptomyces coelicolor Single-stranded DNA-binding Protein

Zoran Štefanić
Dušica Vujaklija
Luka Andrišić
Goran Mikleušević
Miha Andrejašič
Dušan Turk
Marija Luić

Puni tekst: engleski, pdf (234 KB) str. 35-39 preuzimanja: 480* citiraj
APA 6th Edition
Štefanić, Z., Vujaklija, D., Andrišić, L., Mikleušević, G., Andrejašič, M., Turk, D. i Luić, M. (2007). Preliminary Crystallographic Study of Streptomyces coelicolor Single-stranded DNA-binding Protein. Croatica Chemica Acta, 80 (1), 35-39. Preuzeto s https://hrcak.srce.hr/12816
MLA 8th Edition
Štefanić, Zoran, et al. "Preliminary Crystallographic Study of Streptomyces coelicolor Single-stranded DNA-binding Protein." Croatica Chemica Acta, vol. 80, br. 1, 2007, str. 35-39. https://hrcak.srce.hr/12816. Citirano 21.06.2018.
Chicago 17th Edition
Štefanić, Zoran, Dušica Vujaklija, Luka Andrišić, Goran Mikleušević, Miha Andrejašič, Dušan Turk i Marija Luić. "Preliminary Crystallographic Study of Streptomyces coelicolor Single-stranded DNA-binding Protein." Croatica Chemica Acta 80, br. 1 (2007): 35-39. https://hrcak.srce.hr/12816

Sažetak
Single-stranded DNA-binding proteins (SSBs) play a crucial role in DNA processing such as
replication, repair and recombination in all organisms, from bacteria to human. Streptomyces
coelicolor ssb gene was overexpressed in a heterologous host, Escherichia coli NM522. 15 mg
of purified protein from 1 dm3 of culture was obtained in one-step procedure applying Ni2+
chelating chromatography. Among bacterial SSBs with the solved crystal structure, the S.
coelicolor SSB displayed significant sequence similarity with those from Mycobacterium
tuberculosis and Mycobacterium smegmatis, slow growing bacteria with a high GC content.
Moreover, conserved amino acid region that forms additional ß strand in mycobacterial SSBs
was also found in S. coelicolor SSB. The full-length protein readily crystallises in space group
I222 or I212121 with unit-cell parameters a = 100.8, b = 102.1, c = 164.2 Å. The asymmetric
unit is expected to contain four monomers with solvent content of 52–55 %.

Ključne riječi
single-stranded DNA-binding protein; SSB purification; Streptomyces coelicolor; crystallisation

Hrčak ID: 12816

URI
https://hrcak.srce.hr/12816

Posjeta: 751 *