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Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study

Dubravka Krilov ; School of Medicine, Šalata 3, 41001 Zagreb, Croatia
Carol M. Herak-Kramberger ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Tatjana Ukrainczyk ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Janko N. Herak ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (10 MB) str. 409-415 preuzimanja: 119* citiraj
APA 6th Edition
Krilov, D., Herak-Kramberger, C.M., Ukrainczyk, T. i Herak, J.N. (1995). Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study. Croatica Chemica Acta, 68 (2), 409-415. Preuzeto s https://hrcak.srce.hr/136763
MLA 8th Edition
Krilov, Dubravka, et al. "Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study." Croatica Chemica Acta, vol. 68, br. 2, 1995, str. 409-415. https://hrcak.srce.hr/136763. Citirano 01.03.2021.
Chicago 17th Edition
Krilov, Dubravka, Carol M. Herak-Kramberger, Tatjana Ukrainczyk i Janko N. Herak. "Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study." Croatica Chemica Acta 68, br. 2 (1995): 409-415. https://hrcak.srce.hr/136763
Harvard
Krilov, D., et al. (1995). 'Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study', Croatica Chemica Acta, 68(2), str. 409-415. Preuzeto s: https://hrcak.srce.hr/136763 (Datum pristupa: 01.03.2021.)
Vancouver
Krilov D, Herak-Kramberger CM, Ukrainczyk T, Herak JN. Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study. Croatica Chemica Acta [Internet]. 1995 [pristupljeno 01.03.2021.];68(2):409-415. Dostupno na: https://hrcak.srce.hr/136763
IEEE
D. Krilov, C.M. Herak-Kramberger, T. Ukrainczyk i J.N. Herak, "Formation of Free Radicals in Oxidation of Human Low-Density Lipoprotein. An EPR Spin Trapping Study", Croatica Chemica Acta, vol.68, br. 2, str. 409-415, 1995. [Online]. Dostupno na: https://hrcak.srce.hr/136763. [Citirano: 01.03.2021.]

Sažetak
The EPR spin trapping method with PBN as a spin trap was used to study the formation of free radicals in human low-density lipoprotein (LDL) upon ageing at 37 °C. The PBN associated radicals could be observed after 10 - 20 hours of incubation, depending on the pretreatment of LDL and the individual donor. The radicals observed in the first period of time (until about 20 - 25 hours of incubation) are related to the degradation products of PBN. After that, additional radicals associated with lipids are observed.
The presence of EDTA does not prevent the process. The present experiments prove that some relatively fast oxidation process takes place in LDL incubated at physiological temperature. The process is mild, as judged from the essentially unchanged concentration of thiobarbituric acid-reactive substances (TBARS). An active role of the spin trap is not excluded.

Hrčak ID: 136763

URI
https://hrcak.srce.hr/136763

Posjeta: 218 *