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https://doi.org/10.15255/CABEQ.2014.2248

Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation

S. Povolo ; Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, Legnaro, Padova, Italy
M. Basaglia ; Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, Legnaro, Padova, Italy
F. Fontana ; Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell’Università 16, Legnaro, Padova, Italy
A. Morelli ; Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab) – Dept. of Chemistry and Industrial Chemistry- University of Pisa, via Vecchia Livornese, 1291, 56122, S. Piero a Grado, Pisa, Italy
S. Casella ; Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab) – Dept. of Chemistry and Industrial Chemistry- University of Pisa, via Vecchia Livornese, 1291, 56122, S. Piero a Grado, Pisa, Italy

Puni tekst: engleski, pdf (871 KB) str. 67-74 preuzimanja: 2.319* citiraj
APA 6th Edition
Povolo, S., Basaglia, M., Fontana, F., Morelli, A. i Casella, S. (2015). Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation. Chemical and Biochemical Engineering Quarterly, 29 (2), 67-74. https://doi.org/10.15255/CABEQ.2014.2248
MLA 8th Edition
Povolo, S., et al. "Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation." Chemical and Biochemical Engineering Quarterly, vol. 29, br. 2, 2015, str. 67-74. https://doi.org/10.15255/CABEQ.2014.2248. Citirano 22.06.2021.
Chicago 17th Edition
Povolo, S., M. Basaglia, F. Fontana, A. Morelli i S. Casella. "Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation." Chemical and Biochemical Engineering Quarterly 29, br. 2 (2015): 67-74. https://doi.org/10.15255/CABEQ.2014.2248
Harvard
Povolo, S., et al. (2015). 'Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation', Chemical and Biochemical Engineering Quarterly, 29(2), str. 67-74. https://doi.org/10.15255/CABEQ.2014.2248
Vancouver
Povolo S, Basaglia M, Fontana F, Morelli A, Casella S. Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation. Chemical and Biochemical Engineering Quarterly [Internet]. 2015 [pristupljeno 22.06.2021.];29(2):67-74. https://doi.org/10.15255/CABEQ.2014.2248
IEEE
S. Povolo, M. Basaglia, F. Fontana, A. Morelli i S. Casella, "Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation", Chemical and Biochemical Engineering Quarterly, vol.29, br. 2, str. 67-74, 2015. [Online]. https://doi.org/10.15255/CABEQ.2014.2248

Sažetak
PHA production from waste oils or fats requires microorganisms that should be both excellent PHA producers and equipped with enzymatic activities allowing hydrolysation of triglycerides. Unfortunately, microbes with the combination of substrate-utilization and PHA production are not currently available, and the strategies to be adopted are the use of costly commercial enzymes, or genetic modification of microorganisms exhibiting high PHA product yields.
In the present work, after a general investigation on the ability of Cupriavidus necator to grow on a number of fatty substrates, the possibility to enhance PHA production by limiting intracellular depolymerisation, was investigated. By knocking out the related
phaZ1 gene, the construction of C. necator recombinant strains impaired in depolymerase (PhaZ1) activity was achieved. The polymer yield of the recombinant strain was finally compared to that of the parental C. necator DSM 545.

Ključne riječi
poly(hydroxyalkanoates); glycerol; lipase activity; depolymerase; Cupriavidus necator

Hrčak ID: 141890

URI
https://hrcak.srce.hr/141890

Posjeta: 2.713 *