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https://doi.org/10.15255/CABEQ.2014.2022

Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin

K. Dutta ; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
V. Venkata Dasu ; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
B. Mahanty ; Department of Environmental Engineering, INHA University, Incheon 402751, Republic of Korea
A. Anand Prabhu ; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India

Puni tekst: engleski, pdf (457 KB) str. 437-445 preuzimanja: 576* citiraj
APA 6th Edition
Dutta, K., Venkata Dasu, V., Mahanty, B. i Anand Prabhu, A. (2015). Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin. Chemical and Biochemical Engineering Quarterly, 29 (3), 437-445. https://doi.org/10.15255/CABEQ.2014.2022
MLA 8th Edition
Dutta, K., et al. "Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin." Chemical and Biochemical Engineering Quarterly, vol. 29, br. 3, 2015, str. 437-445. https://doi.org/10.15255/CABEQ.2014.2022. Citirano 22.11.2019.
Chicago 17th Edition
Dutta, K., V. Venkata Dasu, B. Mahanty i A. Anand Prabhu. "Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin." Chemical and Biochemical Engineering Quarterly 29, br. 3 (2015): 437-445. https://doi.org/10.15255/CABEQ.2014.2022
Harvard
Dutta, K., et al. (2015). 'Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin', Chemical and Biochemical Engineering Quarterly, 29(3), str. 437-445. https://doi.org/10.15255/CABEQ.2014.2022
Vancouver
Dutta K, Venkata Dasu V, Mahanty B, Anand Prabhu A. Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin. Chemical and Biochemical Engineering Quarterly [Internet]. 2015 [pristupljeno 22.11.2019.];29(3):437-445. https://doi.org/10.15255/CABEQ.2014.2022
IEEE
K. Dutta, V. Venkata Dasu, B. Mahanty i A. Anand Prabhu, "Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin", Chemical and Biochemical Engineering Quarterly, vol.29, br. 3, str. 437-445, 2015. [Online]. https://doi.org/10.15255/CABEQ.2014.2022

Sažetak
Using tomato-peel extracted cutin, an economically viable substrate, cutinase production by Pseudomonas cepacia was studied at different initial substrate concentrations (2–20 g L–1). The highest volumetric enzyme activity was observed at 10 g L–1 of cutin, which was inhibited at further higher concentrations. Various 3-, 4- and 5- parametric Monod-variant models were chosen to analyze the inhibition kinetics. The model parameters as well as goodness of fit were estimated using non-linear regression analysis. The
4- parameter Webb model was the best-fit model (R2 = 0.933), followed by the 3-parameter Andrews model (R2 = 0.92). Parameter sensitivity analysis revealed that the maximum specific growth rate was the most sensitive parameter for both the models, and the
Webb constant was the least sensitive. Finally, based on a strong evidence ratio 190.65 from Akaike’s information content criteria analysis as well as extra sum of square F test (P > 0.05), it was found that 3-parameter Andrews model gave the best fit.

Ključne riječi
cutinase; Pseudomonas cepacia; substrate inhibition model; Akaike’s information content criteria; parameter sensitivity

Hrčak ID: 147009

URI
https://hrcak.srce.hr/147009

Posjeta: 851 *