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Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2014.2079

Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes

M. Jořenek ; Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic
L. Zajoncová ; Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic

Puni tekst: engleski, pdf (821 KB) str. 457-466 preuzimanja: 540* citiraj
APA 6th Edition
Jořenek, M. i Zajoncová, L. (2015). Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes. Chemical and Biochemical Engineering Quarterly, 29 (3), 457-466. https://doi.org/10.15255/CABEQ.2014.2079
MLA 8th Edition
Jořenek, M. i L. Zajoncová. "Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes." Chemical and Biochemical Engineering Quarterly, vol. 29, br. 3, 2015, str. 457-466. https://doi.org/10.15255/CABEQ.2014.2079. Citirano 13.11.2019.
Chicago 17th Edition
Jořenek, M. i L. Zajoncová. "Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes." Chemical and Biochemical Engineering Quarterly 29, br. 3 (2015): 457-466. https://doi.org/10.15255/CABEQ.2014.2079
Harvard
Jořenek, M., i Zajoncová, L. (2015). 'Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes', Chemical and Biochemical Engineering Quarterly, 29(3), str. 457-466. https://doi.org/10.15255/CABEQ.2014.2079
Vancouver
Jořenek M, Zajoncová L. Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes. Chemical and Biochemical Engineering Quarterly [Internet]. 2015 [pristupljeno 13.11.2019.];29(3):457-466. https://doi.org/10.15255/CABEQ.2014.2079
IEEE
M. Jořenek i L. Zajoncová, "Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes", Chemical and Biochemical Engineering Quarterly, vol.29, br. 3, str. 457-466, 2015. [Online]. https://doi.org/10.15255/CABEQ.2014.2079

Sažetak
Laccase from Trametes versicolor was immobilized on magnetic (Fe3O4) nanoparticles and microparticles. Free and immobilized laccase had activities 163 and about 4.6 nkat mg–1 respectively. Immobilized was 125.8 µg enzyme mg–1 of nanoparticles and 13 µg enzyme mg–1 of microparticles. The Km were 10.55, 9.02 and 11.14 mmol L–1 for free laccase, immobilized on nanoparticles and immobilized on microparticles. The pH optimum after immobilization was about 0.5 pH less than before immobilization. Immobilized laccase retained 55 % (nano) and 47 % (micro) of initial activity after nine repetitions. Values of T50 were calculated respectively for free laccase, immobilized on nanoparticles and microparticles at 54.5 °C, 57.5 °C and 46 °C. Dyes Direct Blue 78, Acid Blue 225, Reactive Red 195, Acid Blue 74, and Phenol Red were used for decolorization by free and immobilized laccase. Laccase was able to decolorize most of the dyes well.

Ključne riječi
laccase; immobilization; dyes; biodegradation; magnetic particles

Hrčak ID: 147011

URI
https://hrcak.srce.hr/147011

Posjeta: 734 *