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Arhiv za higijenu rada i toksikologiju, Vol. 39 No. 2, 1988.

Kratko priopćenje

Ispitivanje biosinteze T-2 toksina u čistoj kulturi u različitim laboratorijskim uslovima

A. Bočarov-Stančić ; Institut za biološka istraživanja "Siniša Stanković" Beograd , Srbija
M. Muntanola-Cvetković ; Institut za biološka istraživanja "Siniša Stanković" Beograd , Srbija

Puni tekst: srpski, pdf (5 MB) str. 227-232 preuzimanja: 67* citiraj
APA 6th Edition
Bočarov-Stančić, A. i Muntanola-Cvetković, M. (1988). T-2 toxin biosynthesis in pure cultures under different laboratory conditions. Arhiv za higijenu rada i toksikologiju, 39 (2), 227-232. Preuzeto s https://hrcak.srce.hr/152849
MLA 8th Edition
Bočarov-Stančić, A. i M. Muntanola-Cvetković. "T-2 toxin biosynthesis in pure cultures under different laboratory conditions." Arhiv za higijenu rada i toksikologiju, vol. 39, br. 2, 1988, str. 227-232. https://hrcak.srce.hr/152849. Citirano 17.02.2019.
Chicago 17th Edition
Bočarov-Stančić, A. i M. Muntanola-Cvetković. "T-2 toxin biosynthesis in pure cultures under different laboratory conditions." Arhiv za higijenu rada i toksikologiju 39, br. 2 (1988): 227-232. https://hrcak.srce.hr/152849
Harvard
Bočarov-Stančić, A., i Muntanola-Cvetković, M. (1988). 'T-2 toxin biosynthesis in pure cultures under different laboratory conditions', Arhiv za higijenu rada i toksikologiju, 39(2), str. 227-232. Preuzeto s: https://hrcak.srce.hr/152849 (Datum pristupa: 17.02.2019.)
Vancouver
Bočarov-Stančić A, Muntanola-Cvetković M. T-2 toxin biosynthesis in pure cultures under different laboratory conditions. Arh Hig Rada Toksikol. [Internet]. 1988 [pristupljeno 17.02.2019.];39(2):227-232. Dostupno na: https://hrcak.srce.hr/152849
IEEE
A. Bočarov-Stančić i M. Muntanola-Cvetković, "T-2 toxin biosynthesis in pure cultures under different laboratory conditions", Arhiv za higijenu rada i toksikologiju, vol.39, br. 2, str. 227-232, 1988. [Online]. Dostupno na: https://hrcak.srce.hr/152849. [Citirano: 17.02.2019.]

Sažetak
Seven isolates of the fungal genus Fusarium, comprising three species: F.culmorum, F.sporotrichioides and F.tricinctum, were tested for T-2 toxin production. Cultivation was carried oout concurrently on a natural substrate (wheat grains) under temperature stress conditions (7 days at 28 °C, 21 days at 15-16 °C), and in a semi-synthetic liquid medium (5% glucose, 0.1 % peptone-1, 0.1 % yeast extract) on a rotary shaker (200 rpm, 5 days at 26 °C). Of the fungal isolates cultivated on wheat grains 85.7% were found to be toxicogenic; the yields of T-2 toxin were rather small (12.5-52.5 mg/kg dry weight). In the liquid medium all Fusarium cultures biosynthesized T-2 toxin; the quantities produced ranged from 0.8 to 175.0 mg/l of the filtrate. At least half of the dispensed sugar was present in the medium when T-2 toxin production started. No correlation was found between the type of mycelial growth, sporulation, biomass quantities and the intensity of T-2 toxin biosynthesis. Most of the produced trichothecene was of extracellular origin. Fermentation in the semi-synthetic liquid medium using a rotary shaker was more suitable for screening the toxicity of the fungal isolates in pure cultures because of a shorter period of incubation, simpler sample preparation and T-2 toxin extraction. In this way factors influencing toxin production could be defined more precisely.

Hrčak ID: 152849

URI
https://hrcak.srce.hr/152849

[srpski]

Posjeta: 152 *