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Izvorni znanstveni članak
https://doi.org/10.1515/acph-2016-0026

A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2

SUPHANNEE THANYAPHOO ; Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-yai, Songkhla, Thailand
JASADEE KAEWSRICHAN ; Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, and Nanotec-PSU Center of Excellence on Drug Delivery System, Prince of Songkla University, Hat-yai, Songkhla, Thailand

Puni tekst: engleski, pdf (2 MB) str. 373-385 preuzimanja: 248* citiraj
APA 6th Edition
THANYAPHOO, S. i KAEWSRICHAN, J. (2016). A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2. Acta Pharmaceutica, 66 (3), 373-385. https://doi.org/10.1515/acph-2016-0026
MLA 8th Edition
THANYAPHOO, SUPHANNEE i JASADEE KAEWSRICHAN. "A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2." Acta Pharmaceutica, vol. 66, br. 3, 2016, str. 373-385. https://doi.org/10.1515/acph-2016-0026. Citirano 15.11.2019.
Chicago 17th Edition
THANYAPHOO, SUPHANNEE i JASADEE KAEWSRICHAN. "A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2." Acta Pharmaceutica 66, br. 3 (2016): 373-385. https://doi.org/10.1515/acph-2016-0026
Harvard
THANYAPHOO, S., i KAEWSRICHAN, J. (2016). 'A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2', Acta Pharmaceutica, 66(3), str. 373-385. https://doi.org/10.1515/acph-2016-0026
Vancouver
THANYAPHOO S, KAEWSRICHAN J. A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2. Acta Pharm. [Internet]. 2016 [pristupljeno 15.11.2019.];66(3):373-385. https://doi.org/10.1515/acph-2016-0026
IEEE
S. THANYAPHOO i J. KAEWSRICHAN, "A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2", Acta Pharmaceutica, vol.66, br. 3, str. 373-385, 2016. [Online]. https://doi.org/10.1515/acph-2016-0026

Sažetak
Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42 clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.

Ključne riječi
heparin; carbodiimide; rhBMP2; delivery scaffold; bone tissue engineering

Hrčak ID: 154034

URI
https://hrcak.srce.hr/154034

Posjeta: 373 *