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Acta Pharmaceutica, Vol.66 No.3 Rujan 2016.

Izvorni znanstveni članak
https://doi.org/10.1515/acph-2016-0028

Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

EZZAT ABDEL-LATEEF ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
FATEN MAHMOUD ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
OLFAT HAMMAM ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
EMAN EL-AHWANY ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
EMAN EL-WAKIL ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
SHERIHAN KANDIL ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
HODA ABU TALEB ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
MORTADA EL-SAYED ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt
HANAA HASSENEIN ; Theodor Bilharz Research Institute, Kornaish El-Nile, 12661 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt

Puni tekst: engleski, pdf (881 KB) str. 387-398 preuzimanja: 1.619* citiraj
APA 6th Edition
ABDEL-LATEEF, E., MAHMOUD, F., HAMMAM, O., EL-AHWANY, E., EL-WAKIL, E., KANDIL, S., ... HASSENEIN, H. (2016). Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2). Acta Pharmaceutica, 66 (3), 387-398. https://doi.org/10.1515/acph-2016-0028
MLA 8th Edition
ABDEL-LATEEF, EZZAT, et al. "Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)." Acta Pharmaceutica, vol. 66, br. 3, 2016, str. 387-398. https://doi.org/10.1515/acph-2016-0028. Citirano 19.08.2018.
Chicago 17th Edition
ABDEL-LATEEF, EZZAT, FATEN MAHMOUD, OLFAT HAMMAM, EMAN EL-AHWANY, EMAN EL-WAKIL, SHERIHAN KANDIL, HODA ABU TALEB, MORTADA EL-SAYED i HANAA HASSENEIN. "Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)." Acta Pharmaceutica 66, br. 3 (2016): 387-398. https://doi.org/10.1515/acph-2016-0028
Harvard
ABDEL-LATEEF, E., et al. (2016). 'Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)', Acta Pharmaceutica, 66(3), str. 387-398. doi: https://doi.org/10.1515/acph-2016-0028
Vancouver
ABDEL-LATEEF E, MAHMOUD F, HAMMAM O, EL-AHWANY E, EL-WAKIL E, KANDIL S i sur. Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2). Acta Pharm. [Internet]. 30.09.2016. [pristupljeno 19.08.2018.];66(3):387-398. doi: https://doi.org/10.1515/acph-2016-0028
IEEE
E. ABDEL-LATEEF, et al., "Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)", Acta Pharmaceutica, vol.66, br. 3, str. 387-398, Kolovoz 2018. [Online]. doi: https://doi.org/10.1515/acph-2016-0028

Sažetak
The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 µg mL–1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 µg mL–1). Degenerative and apoptotic changes were more evident in curcumin-treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 µg g–1 dried methanol extract) in C. longa rhizomes.

Ključne riječi
Curcuma longa L.; GC-MS analysis; curcumin; anticancer activity

Hrčak ID: 154045

URI
https://hrcak.srce.hr/154045

Posjeta: 1.808 *