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Izvorni znanstveni članak
https://doi.org/10.5562/cca2806
Simplified Method for Rapid Purification of Soluble Histones
Nives Ivić
; Gene Center Munich and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany
Bernhard Groschup
; Gene Center Munich and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany
Silvija Bilokapić
orcid.org/0000-0003-2199-2929
; Gene Center Munich and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany
Mario Halić
; Gene Center Munich and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany
| Puni tekst: engleski, pdf (3 MB) | str. 153-162 | citiraj | |
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APA 6th Edition Ivić, N., Groschup, B., Bilokapić, S. i Halić, M. (2016). Simplified Method for Rapid Purification of Soluble Histones. Croatica Chemica Acta, 89 (2), 153-162. https://doi.org/10.5562/cca2806 MLA 8th Edition Ivić, Nives, et al. "Simplified Method for Rapid Purification of Soluble Histones." Croatica Chemica Acta, vol. 89, br. 2, 2016, str. 153-162. https://doi.org/10.5562/cca2806. Citirano 09.03.2021. Chicago 17th Edition Ivić, Nives, Bernhard Groschup, Silvija Bilokapić i Mario Halić. "Simplified Method for Rapid Purification of Soluble Histones." Croatica Chemica Acta 89, br. 2 (2016): 153-162. https://doi.org/10.5562/cca2806 Harvard Ivić, N., et al. (2016). 'Simplified Method for Rapid Purification of Soluble Histones', Croatica Chemica Acta, 89(2), str. 153-162. https://doi.org/10.5562/cca2806 Vancouver Ivić N, Groschup B, Bilokapić S, Halić M. Simplified Method for Rapid Purification of Soluble Histones. Croatica Chemica Acta [Internet]. 2016 [pristupljeno 09.03.2021.];89(2):153-162. https://doi.org/10.5562/cca2806 IEEE N. Ivić, B. Groschup, S. Bilokapić i M. Halić, "Simplified Method for Rapid Purification of Soluble Histones", Croatica Chemica Acta, vol.89, br. 2, str. 153-162, 2016. [Online]. https://doi.org/10.5562/cca2806 |
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Sažetak
Functional and structural studies of histone-chaperone complexes, nucleosome modifications, their interactions with remodelers and regulatory proteins rely on obtaining recombinant histones from bacteria. In the present study, we show that co-expression of Xenopus laevis histone pairs leads to production of soluble H2AH2B heterodimer and (H3H4)2 heterotetramer. The soluble histone complexes are purified by simple chromatographic techniques. Obtained H2AH2B dimer and H3H4 tetramer are proficient in histone chaperone binding and histone octamer and nucleosome formation. Our optimized protocol enables rapid purification of multiple soluble histone variants with a remarkable high yield and simplifies histone octamer preparation. We expect that this simple approach will contribute to the histone chaperone and chromatin research.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
histone; nucleosome; chromatin; protein co-expression
Hrčak ID: 159897
URI
https://hrcak.srce.hr/159897
Moj profil
Srce