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ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON

Evangelos ALATSATIANOS
Marin ARDELEAN
Constantin BOTEZ
Mircea SAVATTI
Radu SESTRAŞ
Mirela CORDEA

Puni tekst: engleski, pdf (196 KB) str. 9-16 preuzimanja: 425* citiraj
APA 6th Edition
ALATSATIANOS, E., ARDELEAN, M., BOTEZ, C., SAVATTI, M., SESTRAŞ, R. i CORDEA, M. (2001). ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON. Journal of Central European Agriculture, 2 (1-2), 9-16. Preuzeto s https://hrcak.srce.hr/16007
MLA 8th Edition
ALATSATIANOS, Evangelos, et al. "ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON." Journal of Central European Agriculture, vol. 2, br. 1-2, 2001, str. 9-16. https://hrcak.srce.hr/16007. Citirano 23.10.2021.
Chicago 17th Edition
ALATSATIANOS, Evangelos, Marin ARDELEAN, Constantin BOTEZ, Mircea SAVATTI, Radu SESTRAŞ i Mirela CORDEA. "ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON." Journal of Central European Agriculture 2, br. 1-2 (2001): 9-16. https://hrcak.srce.hr/16007
Harvard
ALATSATIANOS, E., et al. (2001). 'ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON', Journal of Central European Agriculture, 2(1-2), str. 9-16. Preuzeto s: https://hrcak.srce.hr/16007 (Datum pristupa: 23.10.2021.)
Vancouver
ALATSATIANOS E, ARDELEAN M, BOTEZ C, SAVATTI M, SESTRAŞ R, CORDEA M. ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON. Journal of Central European Agriculture [Internet]. 2001 [pristupljeno 23.10.2021.];2(1-2):9-16. Dostupno na: https://hrcak.srce.hr/16007
IEEE
E. ALATSATIANOS, M. ARDELEAN, C. BOTEZ, M. SAVATTI, R. SESTRAŞ i M. CORDEA, "ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON", Journal of Central European Agriculture, vol.2, br. 1-2, str. 9-16, 2001. [Online]. Dostupno na: https://hrcak.srce.hr/16007. [Citirano: 23.10.2021.]

Sažetak
A melon genomic library was used to isolate and characterize a clone of genomic DNA coding for ascorbate
oxidase (AO) which is considered a key enzyme in the biodegradation of ascorbic acid (AA).
The screening of genomic library was performed by using two probes: AO-PCR product and αE/CMAO3. Seven
clones of recombinant phage, with positive signal, were isolated. The extracted DNA was digested with EcoRI,
Sal I and Bam HI and the combinations among these restriction enzymes. Four clones of AO gene were isolated:
AO1; AO2; AO3 and AO4. Since only clone AO4 had not been characterized so far, the sequencing of this clone
was performed. The isolated sequence of DNA has a length of 3072 bp being bordered at both ends by EcoRI
enzymes.
The comparison between genomic DNA for AO4 in melon and DNA for AO in cucumber revealed a high
similarity (94.45%).

Ključne riječi
isolation; sequencing; genomic DNA; ascorbate oxidaze; ascorbic acid; melon

Hrčak ID: 16007

URI
https://hrcak.srce.hr/16007

Posjeta: 688 *