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Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens

Alexius Freyberger
Elke Hartmann
Franc Krötlinger

Puni tekst: engleski, pdf (104 KB) str. 131-139 preuzimanja: 598* citiraj
APA 6th Edition
Freyberger, A., Hartmann, E. i Krötlinger, F. (2005). Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens. Arhiv za higijenu rada i toksikologiju, 56 (2), 131-139. Preuzeto s https://hrcak.srce.hr/141
MLA 8th Edition
Freyberger, Alexius, et al. "Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens." Arhiv za higijenu rada i toksikologiju, vol. 56, br. 2, 2005, str. 131-139. https://hrcak.srce.hr/141. Citirano 21.09.2019.
Chicago 17th Edition
Freyberger, Alexius, Elke Hartmann i Franc Krötlinger. "Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens." Arhiv za higijenu rada i toksikologiju 56, br. 2 (2005): 131-139. https://hrcak.srce.hr/141
Harvard
Freyberger, A., Hartmann, E., i Krötlinger, F. (2005). 'Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens', Arhiv za higijenu rada i toksikologiju, 56(2), str. 131-139. Preuzeto s: https://hrcak.srce.hr/141 (Datum pristupa: 21.09.2019.)
Vancouver
Freyberger A, Hartmann E, Krötlinger F. Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens. Arh Hig Rada Toksikol. [Internet]. 2005 [pristupljeno 21.09.2019.];56(2):131-139. Dostupno na: https://hrcak.srce.hr/141
IEEE
A. Freyberger, E. Hartmann i F. Krötlinger, "Evaluation of the Rodent Hershberger Bioassay Using Three Reference (Anti)Androgens", Arhiv za higijenu rada i toksikologiju, vol.56, br. 2, str. 131-139, 2005. [Online]. Dostupno na: https://hrcak.srce.hr/141. [Citirano: 21.09.2019.]

Sažetak
Under the umbrella of the Organization for Economic Cooperation and Development (OECD) the rodent Hershberger bioassay is being validated as an in vivo screen for the detection of (anti)androgens. As part of the validation work we studied trenbolone (TREN), 1,1-bis-(4-chlorophenyl)-2,2-dichloroethylene (p,p'-DDE) and vinclozolin (VIN). Oral intubation of castrated rats with TREN [0.3-1.5-8-40 mg kg-1 body weight (b.w.)] for ten days increased androgen-sensitive tissue weights (ASTW) at the high dose. p,p'-DDE (5-16-50-160 mg kg-1 b.w.) and VIN (0-3-10-30-100 mg kg-1 b.w.) orally administered for ten days produced a dose-dependent decrease in ASTW in castrated rats subcutaneously supplemented with testosterone propionate (0.4 mg kg-1 b.w.). p,p'-DDE also strongly increased liver weights and induced hepatocellular hypertrophy and thyroid follicular cell hypertrophy that was most likely mediated by liver enzyme induction. Our data strongly suggest that the OECD protocol of the rodent Hershberger bioassay describes a sensitive in vivo screen, capable of detecting weakly active (anti)androgens. Furthermore, our data may also indicate that thyroid effects could be assessed, if the protocol is amended accordingly.

Ključne riječi
in vivo screen; OECD; p,p'-DDE; thyroid; trenbolone; vinclozolin

Hrčak ID: 141

URI
https://hrcak.srce.hr/141

[hrvatski]

Posjeta: 932 *