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Kratko priopćenje
https://doi.org/10.24099/vet.arhiv.160616

Kombinirana primjena dekstrana i fikola smanjuje udio trombocita u populaciji mononuklearnih stanica izdvojenih iz periferne krvi pilića - kratko priopćenje

Mladen Jergović ; Centre for Research and Transfer of Knowledge in Biotechnology, University of Zagreb, Zagreb, Croatia
Gordana Nedeljković ; Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Željko Cvetić ; Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Željko Gottstein ; Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Krešo Bendelja ; Centre for Research and Transfer of Knowledge in Biotechnology, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (5 MB) str. 623-630 preuzimanja: 448* citiraj
APA 6th Edition
Jergović, M., Nedeljković, G., Cvetić, Ž., Gottstein, Ž. i Bendelja, K. (2017). Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear cells - short communication. Veterinarski arhiv, 87 (5), 623-630. https://doi.org/10.24099/vet.arhiv.160616
MLA 8th Edition
Jergović, Mladen, et al. "Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear cells - short communication." Veterinarski arhiv, vol. 87, br. 5, 2017, str. 623-630. https://doi.org/10.24099/vet.arhiv.160616. Citirano 18.10.2019.
Chicago 17th Edition
Jergović, Mladen, Gordana Nedeljković, Željko Cvetić, Željko Gottstein i Krešo Bendelja. "Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear cells - short communication." Veterinarski arhiv 87, br. 5 (2017): 623-630. https://doi.org/10.24099/vet.arhiv.160616
Harvard
Jergović, M., et al. (2017). 'Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear cells - short communication', Veterinarski arhiv, 87(5), str. 623-630. https://doi.org/10.24099/vet.arhiv.160616
Vancouver
Jergović M, Nedeljković G, Cvetić Ž, Gottstein Ž, Bendelja K. Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear cells - short communication. Veterinarski arhiv [Internet]. 2017 [pristupljeno 18.10.2019.];87(5):623-630. https://doi.org/10.24099/vet.arhiv.160616
IEEE
M. Jergović, G. Nedeljković, Ž. Cvetić, Ž. Gottstein i K. Bendelja, "Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear cells - short communication", Veterinarski arhiv, vol.87, br. 5, str. 623-630, 2017. [Online]. https://doi.org/10.24099/vet.arhiv.160616

Sažetak
Metoda razdvajanja gradijentom gustoće pomoću fikola najčešće je korištena metoda za izdvajanje leukocita iz periferne krvi ptica, ali koja rezultira značajnom kontaminacijom trombocitima. Trombociti u populaciji izdvojenih stanica utječu na rezultate fenotipskih (imunofenotipizacija), ali i funkcionalnih testova T limfocita. Koristeći uzorke pune krvi od 10 cijepljenih muških pilića komercijalnog hibrida nesilica razvili smo metodu izdvajanja leukocita kombiniranom primjenom dekstrana i gradijenta gustoće pomoću fikola CD8αex vivo test proliferacije T limfocita pokazao je smanjenu pozadinsku proliferaciju nestimuliranih stanica nakon izdvajanja leukocita kombiniranom primjenom dekstrana i fikola.

Ključne riječi
dekstran; fikol; pilići; trombociti; leukociti

Hrčak ID: 186223

URI
https://hrcak.srce.hr/186223

[engleski]

Posjeta: 530 *