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A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics

M. Protić ; Department of Organic Chemistry and Biochemistry »Ruaer Boskovic« Institute, Bijenicka 54, 41000 Zagreb, Croatia, Yugoslavia
Ž. Kućan ; Department of Organic Chemistry and Biochemistry »Ruaer Boskovic« Institute, Bijenicka 54, 41000 Zagreb, Croatia, Yugoslavia

Puni tekst: engleski, pdf (5 MB) str. 425-435 preuzimanja: 47* citiraj
APA 6th Edition
Protić, M. i Kućan, Ž. (1979). A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics. Croatica Chemica Acta, 52 (4), 425-435. Preuzeto s https://hrcak.srce.hr/195867
MLA 8th Edition
Protić, M. i Ž. Kućan. "A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics." Croatica Chemica Acta, vol. 52, br. 4, 1979, str. 425-435. https://hrcak.srce.hr/195867. Citirano 28.02.2021.
Chicago 17th Edition
Protić, M. i Ž. Kućan. "A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics." Croatica Chemica Acta 52, br. 4 (1979): 425-435. https://hrcak.srce.hr/195867
Harvard
Protić, M., i Kućan, Ž. (1979). 'A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics', Croatica Chemica Acta, 52(4), str. 425-435. Preuzeto s: https://hrcak.srce.hr/195867 (Datum pristupa: 28.02.2021.)
Vancouver
Protić M, Kućan Ž. A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics. Croatica Chemica Acta [Internet]. 1979 [pristupljeno 28.02.2021.];52(4):425-435. Dostupno na: https://hrcak.srce.hr/195867
IEEE
M. Protić i Ž. Kućan, "A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics", Croatica Chemica Acta, vol.52, br. 4, str. 425-435, 1979. [Online]. Dostupno na: https://hrcak.srce.hr/195867. [Citirano: 28.02.2021.]

Sažetak
The effects of several antibiotics on the initial reaction rates
of the polyphenylalanine and polylysine syntheses were measured
in cell-free systems from Escherichia coli MRE600, stimulated by
polyuridilic and polyadenylic acid, respectively. lVIichaelis-Menten
··type of dependence on the tRNA concentration, and a sigmoidal
(possibly cooperative) dependence on the polynucleotide concentration
was obtained. Puromycin and chloramphenicol were competitive
inhibitors with respect to tRNA, while erythromycin,
streptomycin and chlorotetracycline were non-competitive inhibitors.
On the other hand, erythromycin was apparently a competitive
inhibitor with respect to the synthetic polynucleotide, while
other above-mentioned antibiotics showed either uncompetitive,
non-competitive or mixed type of inhibition. This kinetic approach
enabled the calculation of inhibition constants for the examined
antibiotics; they ranged from 2.5 x 10-7 mol dm·3 for erythromycin
and streptomycin, to over 1 · 10·4 mol dm-a for puromycin.

Hrčak ID: 195867

URI
https://hrcak.srce.hr/195867

Posjeta: 91 *