hrcak mascot   Srce   HID

Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2016.1038

Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase

H. Luo ; Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
K. Han ; Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
Y. Chang ; Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
Y. Wang ; Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
S. Tong ; Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
Z. Nie ; Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
X. Wang ; Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
Y. Wei ; Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China
H. Yu ; Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China
Z. Shen ; Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China

Puni tekst: engleski, pdf (1 MB) str. 125-133 preuzimanja: 128* citiraj
APA 6th Edition
Luo, H., Han, K., Chang, Y., Wang, Y., Tong, S., Nie, Z., ... Shen, Z. (2018). Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase. Chemical and Biochemical Engineering Quarterly, 32 (1), 125-133. https://doi.org/10.15255/CABEQ.2016.1038
MLA 8th Edition
Luo, H., et al. "Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase." Chemical and Biochemical Engineering Quarterly, vol. 32, br. 1, 2018, str. 125-133. https://doi.org/10.15255/CABEQ.2016.1038. Citirano 30.03.2020.
Chicago 17th Edition
Luo, H., K. Han, Y. Chang, Y. Wang, S. Tong, Z. Nie, X. Wang, Y. Wei, H. Yu i Z. Shen. "Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase." Chemical and Biochemical Engineering Quarterly 32, br. 1 (2018): 125-133. https://doi.org/10.15255/CABEQ.2016.1038
Harvard
Luo, H., et al. (2018). 'Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase', Chemical and Biochemical Engineering Quarterly, 32(1), str. 125-133. https://doi.org/10.15255/CABEQ.2016.1038
Vancouver
Luo H, Han K, Chang Y, Wang Y, Tong S, Nie Z i sur. Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase. Chemical and Biochemical Engineering Quarterly [Internet]. 2018 [pristupljeno 30.03.2020.];32(1):125-133. https://doi.org/10.15255/CABEQ.2016.1038
IEEE
H. Luo, et al., "Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase", Chemical and Biochemical Engineering Quarterly, vol.32, br. 1, str. 125-133, 2018. [Online]. https://doi.org/10.15255/CABEQ.2016.1038

Sažetak
Numerous compounds, including weak bases (e.g., glucosamine, ethylenediamine, and pyridine) and weak acids (e.g., bicarbonate, acetate, propionate, and butyrate), were found to inhibit the catalysis of cephalosporin C acylase (CCA), which is a recombinant
enzyme expressed in E. coli. Additionally, the protective effect of the inhibitors on free and immobilized CCA against heat treatment was investigated. The inhibitors were added to increase recovery of the activity of the enzyme immobilized by covalent attachment to an epoxy support. The activities of immobilized CCA obtained in the presence of acetate or bicarbonate were 99.2±2.5 U g–1 and 94.1±3.0 U g–1, respectively, which were 31.7 % and 25 % higher, respectively, than that of the control. In addition, the immobilized
CCA exhibited improved thermostability. The half-life of immobilized CCA obtained in the presence of acetate or bicarbonate increased by 190 % and 120 %, respectively, compared to that of immobilized CCA obtained in the absence of an inhibitor.






Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
cephalosporin C acylase; inhibitor; immobilization; thermostability

Hrčak ID: 197250

URI
https://hrcak.srce.hr/197250

Posjeta: 226 *