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https://doi.org/10.5562/cca3357

Identification of Stable and Enantioselective Lipases for Biotechnological Applications

Karl-Erich Jaeger ; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany
Alexander Fulton ; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany
Ulrich Schwaneberg ; RWTH Aachen University, Lehrstuhl für Biotechnologie, Worringer Weg 3, D-52074, Aachen, Germany
Jörg Pietruszka ; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, D-52426 Jülich, Germany
Filip Kovacic   ORCID icon orcid.org/0000-0002-0313-427X ; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany

Puni tekst: engleski, pdf (3 MB) str. 249-253 preuzimanja: 464* citiraj
APA 6th Edition
Jaeger, K., Fulton, A., Schwaneberg, U., Pietruszka, J. i Kovacic, F. (2018). Identification of Stable and Enantioselective Lipases for Biotechnological Applications. Croatica Chemica Acta, 91 (2), 249-253. https://doi.org/10.5562/cca3357
MLA 8th Edition
Jaeger, Karl-Erich, et al. "Identification of Stable and Enantioselective Lipases for Biotechnological Applications." Croatica Chemica Acta, vol. 91, br. 2, 2018, str. 249-253. https://doi.org/10.5562/cca3357. Citirano 07.03.2021.
Chicago 17th Edition
Jaeger, Karl-Erich, Alexander Fulton, Ulrich Schwaneberg, Jörg Pietruszka i Filip Kovacic. "Identification of Stable and Enantioselective Lipases for Biotechnological Applications." Croatica Chemica Acta 91, br. 2 (2018): 249-253. https://doi.org/10.5562/cca3357
Harvard
Jaeger, K., et al. (2018). 'Identification of Stable and Enantioselective Lipases for Biotechnological Applications', Croatica Chemica Acta, 91(2), str. 249-253. https://doi.org/10.5562/cca3357
Vancouver
Jaeger K, Fulton A, Schwaneberg U, Pietruszka J, Kovacic F. Identification of Stable and Enantioselective Lipases for Biotechnological Applications. Croatica Chemica Acta [Internet]. 2018 [pristupljeno 07.03.2021.];91(2):249-253. https://doi.org/10.5562/cca3357
IEEE
K. Jaeger, A. Fulton, U. Schwaneberg, J. Pietruszka i F. Kovacic, "Identification of Stable and Enantioselective Lipases for Biotechnological Applications", Croatica Chemica Acta, vol.91, br. 2, str. 249-253, 2018. [Online]. https://doi.org/10.5562/cca3357

Sažetak
The implementation of biocatalytic reactions in biotechnological processes requires enzymes with suitable properties. Thus, optimization of existing or identification of novel enzymes is needed. For enzyme optimization, various mutagenesis approaches are used yielding libraries with a large number of protein variants. Additionally, novel enzymes can be identified from metagenomic libraries, which contain bacterial clones expressing DNA isolated from different habitats. Activity-based screening of such libraries is needed to identify enzyme candidates of interest. Here, we provide a protocol for screening of regio- and enantioselective lipases stable under industrially relevant conditions.

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
lipases; screening; biotechnology; stability; enantioselectivity; enzyme assays; high throughput

Hrčak ID: 203538

URI
https://hrcak.srce.hr/203538

Posjeta: 663 *