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https://doi.org/10.18054/pb.v120i1.6775

Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran)

Ali Deljou ; College of Agricultural Science, Department of Agricultural Biotechnology, Bu-Ali Sina University, Hamedan, Iran.
Iman Arezi
Morteza Khanahmadi ; Branch of Central Region (Isfahan), Agricultural Biotechnology Research Institute of Iran (ABRII), Isfahan, Iran.

Puni tekst: engleski, PDF (1 MB) str. 11-21 preuzimanja: 774* citiraj
APA 6th Edition
Deljou, A., Arezi, I. i Khanahmadi, M. (2018). Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran). Periodicum biologorum, 120 (1), 11-21. https://doi.org/10.18054/pb.v120i1.6775
MLA 8th Edition
Deljou, Ali, et al. "Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran)." Periodicum biologorum, vol. 120, br. 1, 2018, str. 11-21. https://doi.org/10.18054/pb.v120i1.6775. Citirano 02.12.2020.
Chicago 17th Edition
Deljou, Ali, Iman Arezi i Morteza Khanahmadi. "Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran)." Periodicum biologorum 120, br. 1 (2018): 11-21. https://doi.org/10.18054/pb.v120i1.6775
Harvard
Deljou, A., Arezi, I., i Khanahmadi, M. (2018). 'Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran)', Periodicum biologorum, 120(1), str. 11-21. https://doi.org/10.18054/pb.v120i1.6775
Vancouver
Deljou A, Arezi I, Khanahmadi M. Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran). Periodicum biologorum [Internet]. 2018 [pristupljeno 02.12.2020.];120(1):11-21. https://doi.org/10.18054/pb.v120i1.6775
IEEE
A. Deljou, I. Arezi i M. Khanahmadi, "Scale-up thermostable α-amylase production in lab-scale fermenter using rice husk as an elicitor by Bacillus licheniformis-AZ2 isolated from Qinarje Hot Spring (Ardebil Prov. of Iran)", Periodicum biologorum, vol.120, br. 1, str. 11-21, 2018. [Online]. https://doi.org/10.18054/pb.v120i1.6775

Sažetak

Background and purpose: Amylases are commercially important enzymes with various biotechnological, clinical and medical applications. This study aimed at scaling up α-amylase production elicited by rice husk in stirred-fermenter using Bacillus lichneniformis-AZ2 isolated from Qinarje Hot Spring.


Materials and methods: Effect of temperature, aeration rate and agitation speed on bacterial growth and ɑ-amylase production were investigated under batch fermentation process in a 3-Lit stirred-fermenter. OFAT method was followed to select optimum level of each parameter. Other factors were set upon the results of previous experiments carried out in shake-flask scale.


Results: Maximum α-amylase production of 17.66 ± 0.87 U/mL (2.1 folds more than shake-flask cultures) was achieved in stirred-fermenter with optimized agitation speed of 100 rpm and 1 vvm aeration rate at 37ºC after 60 h of incubation. This time was shorter than the corresponding fermentation time obtained from shake-flask experiments by half. A comparison of kinetic parameters of fermentation in stirred-fermenter and shake-flask cultures revealed that B. licheniformis-AZ2 was more active to synthesize ɑ-amylase in fermenter. In shaken cultures Qx, Qp, Yp/x, µmax, qp and td, were 0.27 (g/L/h), 228.6 (U/L/h), 13.64 (U/g), 0.055 (h-1), 0.76 (U/g/h) and 12.48 h, whereas in stirred-fermenter the above values were 0.40 (g/L/h), 723.1 (U/L/h), 45.17 (U/g), 0.120 (h-1), 5.42 (U/g/h) and 5.78 h, respectively.


Conclusions: SmF in stirred-fermenter is a potential strategy for ɑ-amylase production. Although for commercialization further studies are needed in pilot-scale. Rice husk as a low-cost agro-waste is preferable to use as the carbon and energy sources, which provides a great ɑ-amylase elicitation.

Ključne riječi
Bacillus licheniformis; Agro-waste; Thermostable a-amylase; Scale-up; Aeration; Agitation; Shake flask; Stirred tank fermenter

Hrčak ID: 204225

URI
https://hrcak.srce.hr/204225

Posjeta: 939 *