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Acta clinica Croatica, Vol. 57 No. 2, 2018.

Izvorni znanstveni članak
https://doi.org/10.20471/acc.2018.57.02.06

Izražaj TFF gena i proteina u tumorima dojke

Maja Tolušić Levak ; Department of Histology and Embryology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia;
Martina Mihalj ; Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia;
Ivan Koprivčić ; Department of Anatomy and Neuroscience, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; Department of Surgery, Osijek University Hospital Centre, Osijek, Croatia;
Sanja Novak ; Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Nikola Bijelić ; Department of Histology and Embryology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia;
Mirela Baus Lončar ; Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia;
Tatjana Belovari ; Department of Histology and Embryology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia;
Kristina Kralik ; Department of Medical Statistics and Informatics, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Biljana Pauzar ; Department of Histology and Embryology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek Croatia; Department of Clinical Cytology, Osijek University Hospital Centre, Osijek, CroatiaOsijek, Osijek, Croatia

Puni tekst: engleski, pdf (2 MB) str. 264-277 preuzimanja: 48* citiraj
APA 6th Edition
Tolušić Levak, M., Mihalj, M., Koprivčić, I., Novak, S., Bijelić, N., Baus Lončar, M., ... Pauzar, B. (2018). Differential Expression of TFF Genes and Proteins in Breast Tumors. Acta clinica Croatica, 57 (2), 264-277. https://doi.org/10.20471/acc.2018.57.02.06
MLA 8th Edition
Tolušić Levak, Maja, et al. "Differential Expression of TFF Genes and Proteins in Breast Tumors." Acta clinica Croatica, vol. 57, br. 2, 2018, str. 264-277. https://doi.org/10.20471/acc.2018.57.02.06. Citirano 18.02.2019.
Chicago 17th Edition
Tolušić Levak, Maja, Martina Mihalj, Ivan Koprivčić, Sanja Novak, Nikola Bijelić, Mirela Baus Lončar, Tatjana Belovari, Kristina Kralik i Biljana Pauzar. "Differential Expression of TFF Genes and Proteins in Breast Tumors." Acta clinica Croatica 57, br. 2 (2018): 264-277. https://doi.org/10.20471/acc.2018.57.02.06
Harvard
Tolušić Levak, M., et al. (2018). 'Differential Expression of TFF Genes and Proteins in Breast Tumors', Acta clinica Croatica, 57(2), str. 264-277. doi: https://doi.org/10.20471/acc.2018.57.02.06
Vancouver
Tolušić Levak M, Mihalj M, Koprivčić I, Novak S, Bijelić N, Baus Lončar M i sur. Differential Expression of TFF Genes and Proteins in Breast Tumors. Acta clinica Croatica [Internet]. 2018 [pristupljeno 18.02.2019.];57(2):264-277. doi: https://doi.org/10.20471/acc.2018.57.02.06
IEEE
M. Tolušić Levak, et al., "Differential Expression of TFF Genes and Proteins in Breast Tumors", Acta clinica Croatica, vol.57, br. 2, str. 264-277, 2018. [Online]. doi: https://doi.org/10.20471/acc.2018.57.02.06

Sažetak
Cilj ovoga istraživanja bio je utvrditi razlike u izražaju gena i proteina TFF1, TFF2 i TFF3 u različitim vrstama tumora dojke te ispitati korelacije između gena TFF i vrsta tumora te gena TFF i kliničko-patoloških karakteristika tumora. U studiju su bile uključene 122 ispitanice kojima je kirurški odstranjen tumor dojke. Uzorci su obrađeni metodom qRT-PCR i metodom imunohistokemije. Geni i proteini TFF1 i TFF3 bili su izraženi u tumorima dojke, dok izražaj gena i proteina TFF2 nije otkriven u tumorskom tkivu. TFF1 je bio izraženiji kod dobroćudnih tumora, dok je TFF3 bio izraženiji kod zloćudnih tumora. TFF1 i TFF3 su bili izraženiji u hormonski ovisnim tumorima, tumorima bez metastaza u limfnim čvorovima, tumorima s umjereno visokim izražajem Ki67 i umjereno diferenciranim tumorima. Jaka pozitivna korelacija uočena je između gena TFF1 i TFF3, a oba su negativno korelirala s faktorom Ki67 i stupnjem diferenciranosti tumora. Dobiveni rezultati pokazuju kako bi TFF1 i TFF3 mogli imati ulogu u patogenezi tumora dojke te bi se potencijalno mogli rabiti za određivanje tumorskog statusa i procjenu malignosti tumora.

Ključne riječi
tumorski proteini; dojka, tumori; imunohistokemija; lančana reakcija polimerazom; limfni čvorovi; Ki-67 antigen; biomarkeri

Hrčak ID: 207518

URI
https://hrcak.srce.hr/207518

[engleski]

Posjeta: 118 *