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https://doi.org/10.24141/1/4/2/4

Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards

Mirela Raos   ORCID icon orcid.org/0000-0001-6588-7886 ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia
Jurjana Novoselac ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia
Iva Lucija Burnać ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia
Marija Lukić ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia
Ivana Leskovar ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia
Branka Golubić Ćepulić ; Clinical Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (646 KB) str. 179-186 preuzimanja: 925* citiraj
APA 6th Edition
Raos, M., Novoselac, J., Burnać, I.L., Lukić, M., Leskovar, I. i Golubić Ćepulić, B. (2018). Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards. Journal of Applied Health Sciences = Časopis za primijenjene zdravstvene znanosti, 4 (2), 179-186. https://doi.org/10.24141/1/4/2/4
MLA 8th Edition
Raos, Mirela, et al. "Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards." Journal of Applied Health Sciences = Časopis za primijenjene zdravstvene znanosti, vol. 4, br. 2, 2018, str. 179-186. https://doi.org/10.24141/1/4/2/4. Citirano 20.09.2021.
Chicago 17th Edition
Raos, Mirela, Jurjana Novoselac, Iva Lucija Burnać, Marija Lukić, Ivana Leskovar i Branka Golubić Ćepulić. "Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards." Journal of Applied Health Sciences = Časopis za primijenjene zdravstvene znanosti 4, br. 2 (2018): 179-186. https://doi.org/10.24141/1/4/2/4
Harvard
Raos, M., et al. (2018). 'Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards', Journal of Applied Health Sciences = Časopis za primijenjene zdravstvene znanosti, 4(2), str. 179-186. https://doi.org/10.24141/1/4/2/4
Vancouver
Raos M, Novoselac J, Burnać IL, Lukić M, Leskovar I, Golubić Ćepulić B. Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards. Journal of Applied Health Sciences = Časopis za primijenjene zdravstvene znanosti [Internet]. 2018 [pristupljeno 20.09.2021.];4(2):179-186. https://doi.org/10.24141/1/4/2/4
IEEE
M. Raos, J. Novoselac, I.L. Burnać, M. Lukić, I. Leskovar i B. Golubić Ćepulić, "Evaluation of antibody screening and identification pre-transfusion tests using DG Gel cards", Journal of Applied Health Sciences = Časopis za primijenjene zdravstvene znanosti, vol.4, br. 2, str. 179-186, 2018. [Online]. https://doi.org/10.24141/1/4/2/4

Sažetak
Background
Pre-transfusion tests vary in sensitivity and specificity and should be evaluated before their implementation into routine use. The aim of this study was to evaluate the diagnostic accuracy of the Grifols DG Gel column agglutination system and to compare the data with two other column agglutination systems used in our laboratory: Ortho BioVue and Bio-Rad. Special attention was focused on using more vials of reagent red blood cells by Grifols DG Gel system when compared to other systems in order to investigate whether it increases the sensitivity for clinically significant antibodies.

Methods
All samples were tested in parallel with Grifols DG Gel, Ortho BioVue and Bio-Rad cards according to manufactures’ instructions. Samples were processed through manual instrumentation. Tests were performed by trained and experienced staff. A total of 419 tests were performed on 302 samples.

Results
Concordant results between Grifols DG Gel system and the other two systems were obtained in 93.8% of the tests. For antibody screening by Grifols DG Gel system, sensitivity was 97.53%, specificity was 100%, predictive positive value was 100% and predictive negative value was 97.73%. For antibody identification, the accuracy was 96.03% for Grifols DG Gel system, 97.22% for Ortho BioVue and 94.44% for Bio-Rad.

Conclusions
The Grifols DG gel system shows high diagnostic accuracy and is safe for pre-transfusion compatibility procedures in blood transfusion laboratories. Using more vials of reagent red blood cells by Grifols DG Gel system when compared to other systems increases the sensitivity for some antibody specificities, particularly anti-Jka. This could have major impact on the prevention of delayed transfusion reactions.

Ključne riječi
program evaluation; blood grouping and crossmatching; haemagglutination tests; erythrocytes; isoantibodies

Hrčak ID: 207640

URI
https://hrcak.srce.hr/207640

[hrvatski]

Posjeta: 1.304 *