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Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes

B. Štraus ; Institute of Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia, Yugoslavia
I. Berkeš ; Institute of Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia, Yugoslavia

Puni tekst: engleski, pdf (7 MB) str. 105-111 preuzimanja: 41* citiraj
APA 6th Edition
Štraus, B. i Berkeš, I. (1966). Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes. Croatica Chemica Acta, 38 (2), 105-111. Preuzeto s https://hrcak.srce.hr/208171
MLA 8th Edition
Štraus, B. i I. Berkeš. "Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes." Croatica Chemica Acta, vol. 38, br. 2, 1966, str. 105-111. https://hrcak.srce.hr/208171. Citirano 04.03.2021.
Chicago 17th Edition
Štraus, B. i I. Berkeš. "Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes." Croatica Chemica Acta 38, br. 2 (1966): 105-111. https://hrcak.srce.hr/208171
Harvard
Štraus, B., i Berkeš, I. (1966). 'Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes', Croatica Chemica Acta, 38(2), str. 105-111. Preuzeto s: https://hrcak.srce.hr/208171 (Datum pristupa: 04.03.2021.)
Vancouver
Štraus B, Berkeš I. Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes. Croatica Chemica Acta [Internet]. 1966 [pristupljeno 04.03.2021.];38(2):105-111. Dostupno na: https://hrcak.srce.hr/208171
IEEE
B. Štraus i I. Berkeš, "Transaminating Processes Involving Histidine in Human Haemolysed Erythrocytes", Croatica Chemica Acta, vol.38, br. 2, str. 105-111, 1966. [Online]. Dostupno na: https://hrcak.srce.hr/208171. [Citirano: 04.03.2021.]

Sažetak
Haemolysates of human red blood c ells show transaminating
activity not only between a-ketoglutaric acid and both alanine and
aspartic acid, but with other amino acids not observed previousl y.
The behaviour of h istidine in particular was studied in these
reactions since, by incubation with a-ketoglutaric acid, glutamic
acid was formed, but with pyruvate aspartic acid was unexpectedly
found.
The presence of aspartic acid was demonstrated by paper
chromatography in different solvents and by paper electrophoresis.
Imidazole derivatives were detected with Pauly's reagent.
A reaction scheme is proposed whereby pyruvate enters partly
a transaminating process with histidine, from which the resulting
imidazolyl-pyruvate gives imidazolyl-acetate by decarboxylation.
The carbon dioxide released in this process reacts with pyruvate
in a Wood-Werkmann reaction, giving rise to oxaloacetate, from
which aspartic acid is finally formed by a secondary amino-transfering
reaction with surplus histidine.
Imidazole acetate was detected by paper chromatography, and
imidazole pyruvate by the enol-borate tautomerase m ethod.

Hrčak ID: 208171

URI
https://hrcak.srce.hr/208171

Posjeta: 89 *