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https://doi.org/10.2478/aiht-2018-69-3160

Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration

Željka Kuharić ; Andrija Štampar Teaching Institute for Public Health1, Zagreb, Croatia
Željko Jakopović   ORCID icon orcid.org/0000-0001-6448-484X ; University of Zagreb, Faculty of Food Technology and Biotechnology2, Zagreb, Croatia
Iva Čanak   ORCID icon orcid.org/0000-0003-0973-138X ; University of Zagreb, Faculty of Food Technology and Biotechnology2, Zagreb, Croatia
Jadranka Frece ; University of Zagreb, Faculty of Food Technology and Biotechnology2, Zagreb, Croatia
Jasna Bošnir   ORCID icon orcid.org/0000-0002-9877-2927 ; Andrija Štampar Teaching Institute for Public Health1, Zagreb, Croatia
Željka Pavlek ; Andrija Štampar Teaching Institute for Public Health1, Zagreb, Croatia
Martina Ivešić   ORCID icon orcid.org/0000-0003-1405-6526 ; Andrija Štampar Teaching Institute for Public Health1, Zagreb, Croatia
Ksenija Markov   ORCID icon orcid.org/0000-0001-9188-366X ; University of Zagreb, Faculty of Food Technology and Biotechnology2, Zagreb, Croatia

Puni tekst: engleski, pdf (258 KB) str. 334-339 preuzimanja: 321* citiraj
APA 6th Edition
Kuharić, Ž., Jakopović, Ž., Čanak, I., Frece, J., Bošnir, J., Pavlek, Ž., ... Markov, K. (2018). Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration. Arhiv za higijenu rada i toksikologiju, 69 (4), 334-339. https://doi.org/10.2478/aiht-2018-69-3160
MLA 8th Edition
Kuharić, Željka, et al. "Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration." Arhiv za higijenu rada i toksikologiju, vol. 69, br. 4, 2018, str. 334-339. https://doi.org/10.2478/aiht-2018-69-3160. Citirano 04.08.2020.
Chicago 17th Edition
Kuharić, Željka, Željko Jakopović, Iva Čanak, Jadranka Frece, Jasna Bošnir, Željka Pavlek, Martina Ivešić i Ksenija Markov. "Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration." Arhiv za higijenu rada i toksikologiju 69, br. 4 (2018): 334-339. https://doi.org/10.2478/aiht-2018-69-3160
Harvard
Kuharić, Ž., et al. (2018). 'Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration', Arhiv za higijenu rada i toksikologiju, 69(4), str. 334-339. https://doi.org/10.2478/aiht-2018-69-3160
Vancouver
Kuharić Ž, Jakopović Ž, Čanak I, Frece J, Bošnir J, Pavlek Ž i sur. Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration. Arh Hig Rada Toksikol. [Internet]. 2018 [pristupljeno 04.08.2020.];69(4):334-339. https://doi.org/10.2478/aiht-2018-69-3160
IEEE
Ž. Kuharić, et al., "Removing aflatoxin M1 from milk with native lactic acid bacteria, centrifugation, and filtration", Arhiv za higijenu rada i toksikologiju, vol.69, br. 4, str. 334-339, 2018. [Online]. https://doi.org/10.2478/aiht-2018-69-3160

Sažetak
In order to minimise human exposure to aflatoxin M1 (AFM1) the levels of this highly carcinogenic mycotoxin in milk, heat-treated milk, and other dairy products have been limited to <0.05 μg kg-1. However, its removal from dairy products presents a challenge for dairy producers, as commercial additives change organoleptic properties, and filtration alone yields poor results. The aim of this study was to find a strain of lactic acid bacteria (LAB) from milk or dairy products that most effectively binds AFM1 and to see whether heat treatment of the selected LAB affects the binding efficiency. We also wanted to investigate whether centrifugation can improve filtering of the obtained AFM1-LAB complexes from milk. To do that, we isolated and identified 10 native LAB species/strains, incubated their viable or heat-treated cells (108 CFU mL-1) in milk spiked with 0.5 μg L-1 of AFM1 at 4 °C for 0, 2, 4, and 24 h, and quantified the amount of unbound AFM1 with HPLC. AFM1 binding efficiency ranged from 21 to 92 % for viable cells and from 26 to 94 % for the heattreated ones. Since both viable and heat-treated Lactobacillus plantarum KM showed the best results, we used it for the next step in AFM1 removal from milk. Heat treatment in combination with filtration and centrifugation yielded removal as high as 96 %.

Ključne riječi
HPLC; lactic acid bacteria; L. plantarum KM; secondary fungal metabolites; viable and heat-treated cells

Hrčak ID: 213655

URI
https://hrcak.srce.hr/213655

[hrvatski]

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