Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2018.1389
Effect of Developed Low Cost Minimal Medium on Lipid and Exopolysaccharide Production by Lipomyces starkeyi Under Repeated Fed-batch and Continuous Cultivation
V. Thirumal
; Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA 70503, USA
A. Chistoserdov
; Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70503, USA
R. Bajpai
; Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA 70503, USA
J. Bader
; Beuth University of Applied Sciences, Life Sciences and Technology, Biotechnology, Berlin, Germany
M. K. Popovic
; Beuth University of Applied Sciences, Life Sciences and Technology, Biotechnology, Berlin, Germany
R. Subramaniam
; Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA 70503, USA
Sažetak
The main objective of this study was to investigate the effects of a low cost minimal medium, developed by the UL Bioprocessing Lab, on the cultivation of Lipomyces starkeyi NRRL Y-11557 using repeated fed-batch and continuous fermentation strategies. The highest cell and lipid concentrations obtained were 22.7 g L–1 and 11.67 g L–1 under
repeated fed-batch cultivation, respectively. Continuous cultivation with the dilution rate of 0.06 h–1 presented the highest cell (0.401 g g–1) and lipid yields (0.177g g–1). Exopolysaccharide production was observed when L. starkeyi was cultivated in the minimal media supplemented with 90 g L–1 glucose under repeated fed-batch fermentation. The produced exopolysaccharide is likely composed of 4–5 repeating sugar units, incorporating mannose and galactose and their respective uronic acids.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
minimal medium; Lipomyces starkeyi; lipid; exopolysaccharide; fed-batch mode; continuous mode
Hrčak ID:
215700
URI
Datum izdavanja:
15.1.2019.
Posjeta: 984 *