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Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2018.1409
Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae
N. Marđetko
orcid.org/0000-0002-3857-9818
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
M. Novak
orcid.org/0000-0002-9638-615X
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
A. Trontel
orcid.org/0000-0003-2307-5585
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
M. Grubišić
orcid.org/0000-0001-6056-2951
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
M. Galić
; Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
B. Šantek
orcid.org/0000-0001-9583-534X
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
| Puni tekst: engleski, pdf (2 MB) | str. 483-499 | citiraj | |
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APA 6th Edition Marđetko, N., Novak, M., Trontel, A., Grubišić, M., Galić, M. i Šantek, B. (2018). Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae. Chemical and Biochemical Engineering Quarterly, 32 (4), 483-499. https://doi.org/10.15255/CABEQ.2018.1409 MLA 8th Edition Marđetko, N., et al. "Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae." Chemical and Biochemical Engineering Quarterly, vol. 32, br. 4, 2018, str. 483-499. https://doi.org/10.15255/CABEQ.2018.1409. Citirano 13.08.2020. Chicago 17th Edition Marđetko, N., M. Novak, A. Trontel, M. Grubišić, M. Galić i B. Šantek. "Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae." Chemical and Biochemical Engineering Quarterly 32, br. 4 (2018): 483-499. https://doi.org/10.15255/CABEQ.2018.1409 Harvard Marđetko, N., et al. (2018). 'Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae', Chemical and Biochemical Engineering Quarterly, 32(4), str. 483-499. https://doi.org/10.15255/CABEQ.2018.1409 Vancouver Marđetko N, Novak M, Trontel A, Grubišić M, Galić M, Šantek B. Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae. Chemical and Biochemical Engineering Quarterly [Internet]. 2018 [pristupljeno 13.08.2020.];32(4):483-499. https://doi.org/10.15255/CABEQ.2018.1409 IEEE N. Marđetko, M. Novak, A. Trontel, M. Grubišić, M. Galić i B. Šantek, "Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae", Chemical and Biochemical Engineering Quarterly, vol.32, br. 4, str. 483-499, 2018. [Online]. https://doi.org/10.15255/CABEQ.2018.1409 |
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Sažetak
Sustainable recycling of lignocellulosic biomass includes utilization of all carbohydrates present in its hydrolysates. Since wheat straw is a xylose-rich raw material, utilization of xylose from obtained liquid part (liquor) of hydrolysates improves overall bioprocess efficiency. In this work, dilute acid pre-treatment of wheat straw was performed in high-pressure reactor at different temperatures (160 °C – 200 °C), residence times (1 min – 10 min), and acids (H2SO4 and H3PO4) concentrations. During dilute acid pre-treatment, hemicellulose is degraded to pentose sugars that cannot be used by industrial ethanol-
producing yeasts. Therefore, genetically engineered Saccharomyces cerevisiae strain that can utilize xylose was used. Fermentations were performed on different xylose-rich liquor wheat straw hydrolysates in shake-flasks and in horizontal rotating tubular bioreactor. The efficiency of fermentations carried out in shake flasks using xylose-
rich liquor wheat straw hydrolysates were in the range of 19.61 – 74.51 %. However, the maximum bioprocess efficiency (88.24 %) was observed during fermentation in the HRTB on the liquor wheat straw hydrolysate obtained by pre-treatment with 2 % w/w phosphoric acid.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
dilute acid pre-treatment; high-pressure reactor; wheat straw; xylose utilisation; bioethanol; genetically engineered Saccharomyces cerevisiae
Hrčak ID: 215708
URI
https://hrcak.srce.hr/215708
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