Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae
N. Marđetko
orcid.org/0000-0002-3857-9818
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
M. Novak
orcid.org/0000-0002-9638-615X
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
A. Trontel
orcid.org/0000-0003-2307-5585
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
M. Grubišić
orcid.org/0000-0001-6056-2951
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
M. Galić
; Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
B. Šantek
orcid.org/0000-0001-9583-534X
; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
APA 6th Edition Marđetko, N., Novak, M., Trontel, A., Grubišić, M., Galić, M. i Šantek, B. (2018). Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae. Chemical and Biochemical Engineering Quarterly, 32 (4), 483-499. https://doi.org/10.15255/CABEQ.2018.1409
MLA 8th Edition Marđetko, N., et al. "Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae." Chemical and Biochemical Engineering Quarterly, vol. 32, br. 4, 2018, str. 483-499. https://doi.org/10.15255/CABEQ.2018.1409. Citirano 11.04.2021.
Chicago 17th Edition Marđetko, N., M. Novak, A. Trontel, M. Grubišić, M. Galić i B. Šantek. "Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae." Chemical and Biochemical Engineering Quarterly 32, br. 4 (2018): 483-499. https://doi.org/10.15255/CABEQ.2018.1409
Harvard Marđetko, N., et al. (2018). 'Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae', Chemical and Biochemical Engineering Quarterly, 32(4), str. 483-499. https://doi.org/10.15255/CABEQ.2018.1409
Vancouver Marđetko N, Novak M, Trontel A, Grubišić M, Galić M, Šantek B. Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae. Chemical and Biochemical Engineering Quarterly [Internet]. 2018 [pristupljeno 11.04.2021.];32(4):483-499. https://doi.org/10.15255/CABEQ.2018.1409
IEEE N. Marđetko, M. Novak, A. Trontel, M. Grubišić, M. Galić i B. Šantek, "Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae", Chemical and Biochemical Engineering Quarterly, vol.32, br. 4, str. 483-499, 2018. [Online]. https://doi.org/10.15255/CABEQ.2018.1409
Sažetak Sustainable recycling of lignocellulosic biomass includes utilization of all carbohydrates present in its hydrolysates. Since wheat straw is a xylose-rich raw material, utilization of xylose from obtained liquid part (liquor) of hydrolysates improves overall bioprocess efficiency. In this work, dilute acid pre-treatment of wheat straw was performed in high-pressure reactor at different temperatures (160 °C – 200 °C), residence times (1 min – 10 min), and acids (H2SO4 and H3PO4) concentrations. During dilute acid pre-treatment, hemicellulose is degraded to pentose sugars that cannot be used by industrial ethanol-
producing yeasts. Therefore, genetically engineered Saccharomyces cerevisiae strain that can utilize xylose was used. Fermentations were performed on different xylose-rich liquor wheat straw hydrolysates in shake-flasks and in horizontal rotating tubular bioreactor. The efficiency of fermentations carried out in shake flasks using xylose-
rich liquor wheat straw hydrolysates were in the range of 19.61 – 74.51 %. However, the maximum bioprocess efficiency (88.24 %) was observed during fermentation in the HRTB on the liquor wheat straw hydrolysate obtained by pre-treatment with 2 % w/w phosphoric acid.