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https://doi.org/10.5562/cca3528

Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons

Goran Benković   ORCID icon orcid.org/0000-0002-0995-3452 ; Agency for Medicinal Products and Medical Devices, Ksaverska cesta 4, HR-10000 Zagreb, Croatia
Hrvoje Rimac   ORCID icon orcid.org/0000-0001-7232-6489 ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Chemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Željan Maleš ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, HR-10000 Zagreb, Croatia
Siniša Tomić ; Agency for Medicinal Products and Medical Devices, Ksaverska cesta 4, HR-10000 Zagreb, Croatia
Zoran Lončar ; Clinic of Traumatology, Clinical Hospital Center Sisters of Charity, Draškovićeva 19, HR-10000 Zagreb, Croatia
Mirza Bojić   ORCID icon orcid.org/0000-0002-8381-9577 ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Chemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (1 MB) str. 115-123 preuzimanja: 259* citiraj
APA 6th Edition
Benković, G., Rimac, H., Maleš, Ž., Tomić, S., Lončar, Z. i Bojić, M. (2019). Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons. Croatica Chemica Acta, 92 (1), 115-123. https://doi.org/10.5562/cca3528
MLA 8th Edition
Benković, Goran, et al. "Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons." Croatica Chemica Acta, vol. 92, br. 1, 2019, str. 115-123. https://doi.org/10.5562/cca3528. Citirano 24.02.2021.
Chicago 17th Edition
Benković, Goran, Hrvoje Rimac, Željan Maleš, Siniša Tomić, Zoran Lončar i Mirza Bojić. "Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons." Croatica Chemica Acta 92, br. 1 (2019): 115-123. https://doi.org/10.5562/cca3528
Harvard
Benković, G., et al. (2019). 'Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons', Croatica Chemica Acta, 92(1), str. 115-123. https://doi.org/10.5562/cca3528
Vancouver
Benković G, Rimac H, Maleš Ž, Tomić S, Lončar Z, Bojić M. Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons. Croatica Chemica Acta [Internet]. 2019 [pristupljeno 24.02.2021.];92(1):115-123. https://doi.org/10.5562/cca3528
IEEE
G. Benković, H. Rimac, Ž. Maleš, S. Tomić, Z. Lončar i M. Bojić, "Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons", Croatica Chemica Acta, vol.92, br. 1, str. 115-123, 2019. [Online]. https://doi.org/10.5562/cca3528

Sažetak
One of the most important groups of metabolic enzymes is cytochrome P450 superfamily. These enzymes are important in terms of the catalytic diversity and the large number of xenobiotics that are detoxified or activated by converting to reactive metabolites. Flavonoids are xenobiotics to which humans are exposed through diet. Data on their oxidative metabolism mediated by cytochromes P450 are limited. The aim of this study was to determine the enzymatic kinetics of O-demethylation and aromatic hydroxylation of flavonoid aglycons on recombinant cytochrome P450 enzymes and human liver microsomes systems. The study was performed on ten flavonoids, namely 3,7-dihydroxyflavone, 7-hydroxyflavone, acacetin, apigenin, flavone, galangin, kaempferol, naringenin, sakuranetin, and tangeretin using liquid chromatography coupled with mass spectrometry and UV detector. Most relevant enzyme involved in metabolism of flavonoid aglycons is CYP1A2, and its catalytic effectiveness ranges from 0.5 to 2.9 × 106 M–1 min–1. Having in mind high expression and involvement of CYP1A2 in metabolism of xenobiotics including drugs, and its intraindividual differences in expression and activity, potential of drug-flavonoid competitive interactions/inhibitions should be considered when consuming dietary supplement and foods rich in flavonoids.

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
flavonoids; human liver microsomes; cytochromes P450; enzyme kinetics

Hrčak ID: 221949

URI
https://hrcak.srce.hr/221949

Posjeta: 524 *