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Methods for the isolation and identification of bacteria of the genus Campylobacter - classical and molecular methods (Part I)

Marina Mikulić ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Andrea Humski ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Bela Njari ; Veterinarski Fakultet Sveučilišta u Zagrebu, Hrvatska
Dora Stojević ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Luka Jurinović ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Silvio Špičić ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sanja Duvnjak ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Željko Cvetnić ; Hrvatski veterinarski institut, Zagreb, Hrvatska

Puni tekst: hrvatski, pdf (323 KB) str. 297-303 preuzimanja: 133* citiraj
APA 6th Edition
Mikulić, M., Humski, A., Njari, B., Stojević, D., Jurinović, L., Špičić, S., ... Cvetnić, Ž. (2017). Metode izdvajanja i dokazivanja bakterija roda Campylobacter – klasične i molekularne metode (I. dio) . Veterinarska stanica, 48 (4), 297-303. Preuzeto s https://hrcak.srce.hr/222557
MLA 8th Edition
Mikulić, Marina, et al. "Metode izdvajanja i dokazivanja bakterija roda Campylobacter – klasične i molekularne metode (I. dio) ." Veterinarska stanica, vol. 48, br. 4, 2017, str. 297-303. https://hrcak.srce.hr/222557. Citirano 09.07.2020.
Chicago 17th Edition
Mikulić, Marina, Andrea Humski, Bela Njari, Dora Stojević, Luka Jurinović, Silvio Špičić, Sanja Duvnjak i Željko Cvetnić. "Metode izdvajanja i dokazivanja bakterija roda Campylobacter – klasične i molekularne metode (I. dio) ." Veterinarska stanica 48, br. 4 (2017): 297-303. https://hrcak.srce.hr/222557
Harvard
Mikulić, M., et al. (2017). 'Metode izdvajanja i dokazivanja bakterija roda Campylobacter – klasične i molekularne metode (I. dio) ', Veterinarska stanica, 48(4), str. 297-303. Preuzeto s: https://hrcak.srce.hr/222557 (Datum pristupa: 09.07.2020.)
Vancouver
Mikulić M, Humski A, Njari B, Stojević D, Jurinović L, Špičić S i sur. Metode izdvajanja i dokazivanja bakterija roda Campylobacter – klasične i molekularne metode (I. dio) . Veterinarska stanica [Internet]. 2017 [pristupljeno 09.07.2020.];48(4):297-303. Dostupno na: https://hrcak.srce.hr/222557
IEEE
M. Mikulić, et al., "Metode izdvajanja i dokazivanja bakterija roda Campylobacter – klasične i molekularne metode (I. dio) ", Veterinarska stanica, vol.48, br. 4, str. 297-303, 2017. [Online]. Dostupno na: https://hrcak.srce.hr/222557. [Citirano: 09.07.2020.]

Sažetak
Bacteria of the genus Campylobacter require microaerophilic conditions for growth. Use of recognized standardized protocols and prescribed highly selective liquid and/ or solid media is appropriate for routine analysis with classical methods. After the growth of characteristic colonies, microscopic, phenotype and biochemical methods, immunoenzyme tests, resistotypization and/ or mass spectrometry are applied to determine the Campylobacter species. Two classical serotyping methods are used to differentiate Campylobacter strains. There are various PCR protocols intended for the detection of Campylobacter genus, species or subspecies from food samples, environment or faeces. The main advantages of PCR methods are their speed, sensitivity and specificity. The deficiency of this method is that it does not differentiate viable from dead cells, therefore new protocols based on RT-PCR, NASBA and QRT-PRC molecular methods are under development.

Ključne riječi
Campylobacter spp.; bacterial isolation; species determination; standardized methods; PCR

Hrčak ID: 222557

URI
https://hrcak.srce.hr/222557

[hrvatski]

Posjeta: 179 *