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Methods for isolation and identification of bacteria of the genus Campylobacter - genotyping methods (Part II)

Marina Mikulić ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Andrea Humski ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Bela Njari ; Veterinarski Fakultet Sveučilišta u Zagrebu, Hrvatska
Dora Stojević ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Luka Jurinović ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Silvio Spičić ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sanja Duvnjak ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Željko Cvetnić ; Hrvatski veterinarski institut, Zagreb, Hrvatska

Puni tekst: hrvatski, pdf (356 KB) str. 357-363 preuzimanja: 98* citiraj
APA 6th Edition
Mikulić, M., Humski, A., Njari, B., Stojević, D., Jurinović, L., Spičić, S., ... Cvetnić, Ž. (2017). Metode izdvajanja i dokazivanja bakterija roda Campylobacter – metode genotipizacije (II. dio) . Veterinarska stanica, 48 (5), 357-363. Preuzeto s https://hrcak.srce.hr/222644
MLA 8th Edition
Mikulić, Marina, et al. "Metode izdvajanja i dokazivanja bakterija roda Campylobacter – metode genotipizacije (II. dio) ." Veterinarska stanica, vol. 48, br. 5, 2017, str. 357-363. https://hrcak.srce.hr/222644. Citirano 02.07.2020.
Chicago 17th Edition
Mikulić, Marina, Andrea Humski, Bela Njari, Dora Stojević, Luka Jurinović, Silvio Spičić, Sanja Duvnjak i Željko Cvetnić. "Metode izdvajanja i dokazivanja bakterija roda Campylobacter – metode genotipizacije (II. dio) ." Veterinarska stanica 48, br. 5 (2017): 357-363. https://hrcak.srce.hr/222644
Harvard
Mikulić, M., et al. (2017). 'Metode izdvajanja i dokazivanja bakterija roda Campylobacter – metode genotipizacije (II. dio) ', Veterinarska stanica, 48(5), str. 357-363. Preuzeto s: https://hrcak.srce.hr/222644 (Datum pristupa: 02.07.2020.)
Vancouver
Mikulić M, Humski A, Njari B, Stojević D, Jurinović L, Spičić S i sur. Metode izdvajanja i dokazivanja bakterija roda Campylobacter – metode genotipizacije (II. dio) . Veterinarska stanica [Internet]. 2017 [pristupljeno 02.07.2020.];48(5):357-363. Dostupno na: https://hrcak.srce.hr/222644
IEEE
M. Mikulić, et al., "Metode izdvajanja i dokazivanja bakterija roda Campylobacter – metode genotipizacije (II. dio) ", Veterinarska stanica, vol.48, br. 5, str. 357-363, 2017. [Online]. Dostupno na: https://hrcak.srce.hr/222644. [Citirano: 02.07.2020.]

Sažetak
Distinction of bacterial strains below the species or subspecies level is made possible by diverse genotyping methods. Many public health laboratories use pulsed field gel electrophoresis (PFGE), which has been constantly improving since the 1980s. Other methods used include restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) method and ribotyping. The process of determining the order of nucleotides within DNA is called sequencing. Newly evolved methods based on multilocus sequence typing are MLST, rMLST
and cgMLST. These examine the relationships between bacteria of the genue Campylobacter, especially C. jejuni and C. coli. They are useful in investigating possible sources and reservoirs of disease, their connection and the molecular epidemiology of diseases. A new revolutionary method in microbiology is whole genome sequencing (WGS), and it is believed that this method will gradually replace other genotyping methods.

Ključne riječi
Campylobacter spp.; strain differentiation; sequence typing; PFGE

Hrčak ID: 222644

URI
https://hrcak.srce.hr/222644

[hrvatski]

Posjeta: 139 *