Biochemia Medica, Vol. 29 No. 3, 2019.
Izvorni znanstveni članak
https://doi.org/10.11613/BM.2019.030708
Time-dependent variation of ionized calcium in serum samples
Antonija Perović
; Department of Laboratory Diagnostics, Dubrovnik General Hospital, Dubrovnik, Croatia
Marina Njire Bratičević
; Department of Laboratory Diagnostics, Dubrovnik General Hospital, Dubrovnik, Croatia
Sažetak
Introduction: The aim of this study was to compare ionized calcium (iCa) concentrations in arterial heparinized blood and venous serum and to
investigate time-dependent variation of iCa in serum samples centrifuged and analysed at different times.
Materials and methods: Ionized calcium was measured (N = 25) in arterial blood within 20 min after puncture, and in serum within 10 min after
centrifugation conducted 30 min after sampling. Effect of time between sampling and centrifugation was examined in three tubes (N = 30) centrifuged
15, 30 and 60 min after sampling, and analysed within 10 min. Effect of time between centrifugation and analysis was investigated in three
tubes (N = 31) centrifuged 30 min after sampling and analysed: 0-10, 30-40 and 90-100 min after centrifugation. Ionized calcium was measured on
the Siemens RapidLab 348EX analysers. Statistical significance was tested using Wilcoxon test and ANOVA analysis. Clinical significance was judged
against reference change values (RCV).
Results: No statistically significant difference was found between iCa in arterial blood and serum (P = 0.274). A statistically significant decrease was
found: in tubes centrifuged 60 and 15 min after sampling versus 30 min (P = 0.005, P = 0.003); and in tubes analysed 30-40 and 90-100 min after
centrifugation versus 0-10 min (P = 0.021, P = 0.027). Clinically significant changes were observed: 60 versus 30 min (centrifugation) and 90-100
versus 0-10 and 30-40 min (analysis).
Conclusions: Timely analysed arterial blood and serum samples can be used interchangeably. To avoid clinically significant variations, serum tubes
should be centrifuged within 30 min after sampling, and analysis should be performed within 30 min after centrifugation.
Ključne riječi
ionized calcium; serum; heparin; specimen handling; pre-analytical phase
Hrčak ID:
226454
URI
Datum izdavanja:
15.10.2019.
Posjeta: 826 *