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https://doi.org/10.2478/acph-2021-0004

Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus

QIANQIAN LI ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China
LI SHI ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China
YINGYING LIU ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China
SHIMIN GUAN ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China
SHUO ZHANG ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China
BAOGUO CAI ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China
SHAOFENG RONG ; Department of Bioengineering, Shanghai Institute of Technology, Fengxian, Shanghai, 201418, China

Puni tekst: engleski, pdf (1 MB) str. 99-114 preuzimanja: 75* citiraj
APA 6th Edition
LI, Q., SHI, L., LIU, Y., GUAN, S., ZHANG, S., CAI, B. i RONG, S. (2021). Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus. Acta Pharmaceutica, 71 (1), 99-114. https://doi.org/10.2478/acph-2021-0004
MLA 8th Edition
LI, QIANQIAN, et al. "Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus." Acta Pharmaceutica, vol. 71, br. 1, 2021, str. 99-114. https://doi.org/10.2478/acph-2021-0004. Citirano 24.11.2020.
Chicago 17th Edition
LI, QIANQIAN, LI SHI, YINGYING LIU, SHIMIN GUAN, SHUO ZHANG, BAOGUO CAI i SHAOFENG RONG. "Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus." Acta Pharmaceutica 71, br. 1 (2021): 99-114. https://doi.org/10.2478/acph-2021-0004
Harvard
LI, Q., et al. (2021). 'Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus', Acta Pharmaceutica, 71(1), str. 99-114. https://doi.org/10.2478/acph-2021-0004
Vancouver
LI Q, SHI L, LIU Y, GUAN S, ZHANG S, CAI B i sur. Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus. Acta Pharm. [Internet]. 2021 [pristupljeno 24.11.2020.];71(1):99-114. https://doi.org/10.2478/acph-2021-0004
IEEE
Q. LI, et al., "Improved 11α-hydroxycanrenone production by modification of cytochrome P450 monooxygenase gene in Aspergillus ochraceus", Acta Pharmaceutica, vol.71, br. 1, str. 99-114, 2021. [Online]. https://doi.org/10.2478/acph-2021-0004

Sažetak
Eplerenone is a drug that protects the cardiovascular system. 11α-Hydroxycanrenone is a key intermediate in eplerenone synthesis. We found that although the cytochrome P450 (CYP) enzyme system in Aspergillus ochraceus strain MF018 could catalyse the conversion of canrenone to 11α-hydroxycanrenone, its biocatalytic efficiency is low. To improve the efficiency of 11α-hydroxycanrenone production, the CYP monooxygenase-coding gene of MF018 was predicted and cloned based on whole-genome sequencing results. A recombinant A. ochraceus strain MF010 with the high expression of CYP monooxygenase was then obtained through homologous recombination. The biocatalytic rate of this recombinant strain reached 93 % at 60 h without the addition of organic solvents or surfactants and was 17–18 % higher than that of the MF018 strain. Moreover, the biocatalytic time of the MF010 strain was reduced by more than 30 h compared with that of the MF018 strain. These results show that the recombinant A. ochraceus strain MF010 can overcome the limitation of substrate biocatalytic efficiency and thus holds a high potential for application in the industrial production of eplerenone.

Ključne riječi
Aspergillus ochraceus; P450 monooxygenase; homologous recombination; canrenone; 11α-hydroxycanrenone

Hrčak ID: 236062

URI
https://hrcak.srce.hr/236062

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