Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2019.1718
An Efficient Biosurfactant by Pseudomonas stutzeri Z12 Isolated from an Extreme Environment for Remediation of Soil Contaminated with Hydrocarbons
S. Pourfadakari
; a)Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; c)Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
S. Jorfi
; b)Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran c)Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
S. Ghafari
; Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Sažetak
Capability of a biosurfactant produced by Pseudomonas stutzeri Z12 for the removal of hydrocarbons from oily sludge contaminated soil was investigated. The effect of operating parameters, including pH, critical micelle concentration (CMC), salinity, and contact time were studied. The chemical structure of produced biosurfactant was characterized using FTIR and LC-MS-MS analysis, which revealed that the extracted biosurfactant was a combination of both mono- and di-rhamnolipid congeners. The main three congeners RhaC12:1C10 (529.9 m z–1), RhaC12C10 (531 m z–1), and RhaC10C10 (503.2 m z–1) were associated to mono-rhamnolipid, while five congeners, RhaRhaC10C8 (621.2 m z–1), RhaRhaC12C12 (707.7), RhaRhaC10C12 (677.1), RhaRhaC10C12:1 (675.3 m z–1), and RhaRhaC10C1 (649.5 m z–1) were associated to di-rhamnolipid structures. The critical micelle
concentration (CMC) was 80 mg L–1, and emulsification index (E24) values for n-hexadecane, n-hexane, kerosene, diesel oil, xylene, and crude oil were 62.1, 57.6, 54.4, 41.5, 46.9, and 30.2 %, respectively.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
soil washing; biosurfactant; TPH (total petroleum hydrocarbons); oily sludge; soil; Pseudomonas stutzeri Z12
Hrčak ID:
237050
URI
Datum izdavanja:
19.4.2020.
Posjeta: 1.049 *