Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2020.61.55
The effects of daylight exposure on melatonin levels, Kiss1 expression, and melanoma formation in mice
Percin Pazarci
orcid.org/0000-0002-3708-0054
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Halil Kaplan
; Department of Pharmacology, Çukurova University Faculty of Medicine, Adana, Turkey
Davut Alptekin
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Mehmet Yilmaz
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Umit Lüleyap
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Ergin Singirik
; Department of Pharmacology, Çukurova University Faculty of Medicine, Adana, Turkey
Aykut Pelit
; Department of Biophysics Çukurova University Faculty of Medicine, Adana, Turkey
Halil Kasap
; Department of Medical Biology, Çukurova University Faculty of Medicine, Adana, Turkey
Arash Yegani
; Department of Pharmacology and Toxicology, Hatay Mustafa Kemal University Faculty of Veterinary Medicine, Antakya, Turkey
Sažetak
Aim To determine how daylight exposure in mice affects melatonin protein expression in blood and Kiss1 gene expression in the hypothalamus. The second aim was to assess the relationship between skin cancer formation, daylight exposure, melatonin blood level, and kisspeptin gene expression level.
Methods New-born mice (n = 96) were assigned into the blind group or daylight group. The blind group was raised in the dark and the daylight group was raised under 12 hours light/12 hours dark cycle for 17 weeks. At the end of the 11th week, melanoma cell line was inoculated to mice, and tumor growth was observed for 6 weeks. At the end of the experiment, melatonin level was measured from blood serum and Kiss1 expression from the hypothalamus. Results The blind group had significantly higher melatonin and lower Kiss1 expression levels than the daylight group. Tumor volume was inversely proportional to melatonin levels and directly proportional to Kiss1 expression levels. Tumor growth speed was lower in the blind than in the daylight group.
Conclusion Melatonin and Kiss1 were shown to be nvolved in tumor suppression. They were affected by daylight and were mutually affected by each other.
Ključne riječi
Hrčak ID:
240157
URI
Datum izdavanja:
15.2.2020.
Posjeta: 621 *