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Pregledni rad
https://doi.org/10.20471/acc.2020.59.03.12

Uloga novih tehnologija u određivanju proteoma sline nakon postavljanja fiksnog ortodontskog aparata - razvoj novih dijagnostičkih i terapeutskih pristupa

Vojka Zgombić Popović   ORCID icon orcid.org/0000-0002-9355-3290 ; Orthonova Dental Polyclinic, Zagreb, Croatia
Lovorka Grgurević   ORCID icon orcid.org/0000-0002-9814-3178 ; Drago Perović Department of Anatomy, School of Medicine, University of Zagreb, Zagreb, Croatia; Center for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia
Vladimir Trkulja ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Ruđer Novak   ORCID icon orcid.org/0000-0002-5920-4169 ; Center for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia
Dubravka Negovetić-Vranić ; Department of Pediatric and Preventive Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (483 KB) str. 480-488 preuzimanja: 96* citiraj
APA 6th Edition
Zgombić Popović, V., Grgurević, L., Trkulja, V., Novak, R. i Negovetić-Vranić, D. (2020). The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures. Acta clinica Croatica, 59. (3.), 480-488. https://doi.org/10.20471/acc.2020.59.03.12
MLA 8th Edition
Zgombić Popović, Vojka, et al. "The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures." Acta clinica Croatica, vol. 59., br. 3., 2020, str. 480-488. https://doi.org/10.20471/acc.2020.59.03.12. Citirano 23.09.2021.
Chicago 17th Edition
Zgombić Popović, Vojka, Lovorka Grgurević, Vladimir Trkulja, Ruđer Novak i Dubravka Negovetić-Vranić. "The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures." Acta clinica Croatica 59., br. 3. (2020): 480-488. https://doi.org/10.20471/acc.2020.59.03.12
Harvard
Zgombić Popović, V., et al. (2020). 'The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures', Acta clinica Croatica, 59.(3.), str. 480-488. https://doi.org/10.20471/acc.2020.59.03.12
Vancouver
Zgombić Popović V, Grgurević L, Trkulja V, Novak R, Negovetić-Vranić D. The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures. Acta clinica Croatica [Internet]. 2020 [pristupljeno 23.09.2021.];59.(3.):480-488. https://doi.org/10.20471/acc.2020.59.03.12
IEEE
V. Zgombić Popović, L. Grgurević, V. Trkulja, R. Novak i D. Negovetić-Vranić, "The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures", Acta clinica Croatica, vol.59., br. 3., str. 480-488, 2020. [Online]. https://doi.org/10.20471/acc.2020.59.03.12

Sažetak
Ljudska slina obiluje proteinima raznolikih funkcija (enzimi, imunoglobulini, citokini) i podrijetla (krvna plazma, žlijezde slinovnice, mikroflora usne šupljine). Dinamika i volumen izlučene sline kao i njezin sastav (elektroliti, pH, proteini) promjenjivi su tijekom dana, u različitim fiziološkim i patofiziološkim stanjima, lokalnim ali i sustavnim. Stoga se sastav sline, osobito proteinski, intenzivno istražuje u smislu identifikacije potencijalnih biljega i/ili mehanizama sustavnih i lokalnih
bolesti. Nove tehnike analize proteina (tzv. proteomske tehnike) u biološkim tekućinama omogućile su velik napredak u stabilizaciji proteina sline (temeljni uvjet za njihovu analizu) te otkrivanje i onih koji se u slini nalaze u vrlo malim koncentracijama, uključujući i male proteine i peptide. U ovom članku prikazana su temeljna svojstva suvremenih proteomskih tehnika poput tekućinske kromatografije povezane s masenom spektrometrijom (LC-MS), dvodimenzionalne elektroforezemasene spektrometrije (2-DE-MS) ili surface-enhaced laser desorption/ionization/time of flight/mass spectrometry (SELDITOF-
MS). One omogućuju simultano traženje/identifikaciju stotina i tisuća proteinskih molekula i pružaju mogućnost identifikacije onih od potencijalne biološke važnosti u pojedinim stanjima. Ovaj pregled literature usredotočen je na dosadašnje znanje i mogućnosti koje proteomske tehnike pružaju u analizi učinaka ortodontskih aparata na proteinski sastav sline i potragu za potencijalnim biljezima (ne)uspješnosti liječenja ili izvršnim molekulama putem kojih ovaj terapijski postupak ostvaruje učinke.

Ključne riječi
Slina; Proteomika; Ortodontski aparat; Proteini sline

Hrčak ID: 247461

URI
https://hrcak.srce.hr/247461

[engleski]

Posjeta: 320 *